A Convenient Method for the Evaluation of Anti-Tumor Agents Affecting the Cell Cycle
スポンサーリンク
概要
- 論文の詳細を見る
Mitotic index(MI), defined as the ratio(%) of the mitotic-phase-arrested cell number to the total cell number, is higher in the presence of colcemid than in its absence. MI is reduced by anti-tumor agents used in conjunction with colcemid. The ratio of MI, which is defined by the dose of colcemid to that of the supplement with etoposide as an anti-tumor agent and is designated MIR, was 10% at an exposure of 2 h, suggesting that etoposide blocks the cell cycle before 2 h of the mitotic phase. Thus, we established a convenient method to identify a target point in the cell cycle for anti-tumor agents using colcemid. RBL-2H3, a mammalian cell line, exhibited a large number of 2-fold swollen nuclear envelopes after exposure to etoposide. The G_2-index, which is the ratio of the number of swollen nuclei to the total number of nuclei, was higher when the cells were incubated with etoposide than without it. In this study, we suggest that the measurement of the G_2-index contributes to the screening of putative anti-tumor agents.
- 社団法人日本生物工学会の論文
- 2000-11-25
著者
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MASUJIMA Tsutomu
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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IKEDA Kayo
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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OZAWA Koichiro
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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Ikeda Kayo
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Ozawa Koichiro
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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杉山 政則
広島大学医学部総合薬学科
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Sugiyama Masanori
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Sugiyama M
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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PANT BIJAYA
Institute of Pharmaceutical Sciences, Faculty of Medicine, Hiroshima University
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MISHIRO AKIKO
Institute of Pharmaceutical Sciences, Faculty of Medicine, Hiroshima University
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Ikeda Katsunori
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Masujima Tsutomu
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Masujima Tsutomu
Institue Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Sugiyama Masanori
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Sugiyama Masanori
Inst. Pharm. Sci. Hiroshima Univ. Sch. Med.
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Sugiyama Masanori
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Pant Bijaya
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Mishiro Akiko
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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杉山 政則
広島大院・医歯薬
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Masujima T
Hiroshima Univ. School Of Medicine Hiroshima‐shi Jpn
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Masujima T
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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