放線菌の抗生物質耐性機構に関する研究
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概要
- 論文の詳細を見る
Microorganisms which produce antibiotics must be protected from the lethal effects of their own products. The present paper reviews several of our studies concerning self-resistance mechanisms in actinomycetes which produce antibiotic inhibitors of protein synthesis : i) Establishment of an in vitro protein-synthesizing system in Streptomyces. ii) Investigation of the inhibitory effect of their own antibiotics on in vitro protein synthesis. As a result, it was shown that throuth the producers of streptmycin, neomycin, streptothricin, puromycin and blasticidin S all possessed drug-sensitive ribosomes, the protein syntheses could be protected from the action of the drugs by phosphorylation or acetylation of thier own antibiotics. iii) In streptomycin(SM)-producing Streptomyces griseus HUT6037,the proposal of another SM-resistant mechanism in additon to the mechanism of inactivation of SM with SM 6-phosphotransferase. Our study strongly suggested that a decrease in cell permeability to SM which develops with cell growth plays a role in the self-protection. iv) Purification and properties of the antibiotic inactivating enzymes (streptomycin 6-phosphotransferase, puromycin 2"-N-acetyltransferase and blasticidin S acetyltransferase)which play an important role in self-protection of the producers. v) Cloning of two genes coding for the streptomycin-phosphorylating enzymes from S. griseus HUT6037. vi) Analysis of the finding that when protoplasts of streptomycin-sensitive S. griseus KSN were regenerated to the mycelium form, SM-resistant colonies appeared at a high frequency. The resistance mechanism of these regenerated cultures was different from that of ancestral SM-producing S. griseus HUT6037,from with the parent S. griseus KSN was derived. While the resistance to SM of the HUT6037 strain was due mainly to the SM-phosphorylating activity of SM 6-phosphotransferase produced in the cells, the SM-resistant cultures regenerated from protoplasts produced no SM 6-phosphotransferase but the ribosomes changed and became resistant to SM.
- 社団法人日本生物工学会の論文
- 1991-07-25
著者
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杉山 政則
広島大学医学部
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杉山 政則
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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杉山 政則
広島大学医学部総合薬学科
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