Novel Assay System Favorable for the Study of Cell-to-Cell Transmission of HIV-1 and Its Application to the Evaluation of Anti-HIV Drugs

概要

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The cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) was studied using MOLT-4 cells chronically infected with a variant strain of HIV-1_<SF-2> (MOLT-4/HIV-1_<SF-2H>) and CD4^+ human lymphoid MT-4 cells. MOLT-4/HIV-1_<SF-2H> cells produced less than 1 TCID_<50> infectious particles per 10^5 cells per day as determined by the cytopathogenicity in MT-4 cells. However, the expression of envelope glycoproteins gp120 and gp41 on the MOLT-4/HIV-1_<SF-2H> cell membrane was satisfactory for syncytium formation with the uninfected MOLT-4 cells. When MOLT-4/HIV-1_<SF-2H> and MT-4 cells were co-cultured, severe cytopathogenicity was observed in MT-4 cells without being accompanied by the formation of multi-nucleated cells. Thus, the system consisting of MOLT-4/HIV-1_<SF-2H> and MT-4 cells is convenient for exclusive study of the mechanism of cell-to-cell transmission of HIV-1. Using various compounds, it was confirmed that cell-to-cell transmission required both gp120/gp41-CD4 binding and de novo DNA synthesis.
1995-06-15