Molecular Cloning and Expression of a Streptomyces Sarcosine Oxidase Gene in Streptomyces lividans
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概要
- 論文の詳細を見る
A genomic library of Streptomyces sp. KB210-8SY, prepared in the plasmid vector pACYC184,was screened to obtain the gene encoding sarcosine oxidase with probes based on the amino acid sequence of the protein. A plasmid pSOXS13,which was isolated from a clone identified by hybridization with the probes, contained a 8.4-kb insert of Streptomyces DNA. When the 2.0-kb MIuI/EcoRV DNA fragment of pSOXS13 was inserted into the Streptomyces vector pIJ680 and introduced into S. lividans, the transformants produced 100-fold more sarcosine oxidase intracellularly than KB210-8SY. The nucleotides of the 1.7-kb fragment containing the sarcosine oxidase gene were sequenced. An open reading frame encoded a mature sarcosine oxidase consisting of 388 amino acids, with a calculated molecular mass of 42,107 daltons.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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Inouye Yoshio
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine:(present Address)toho U
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Sugiyama M
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Sugiyama Masanori
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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SUZUKI Koji
Research Laboratories, Diagnostic Division, Toyo Jozo Co., Ltd.
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OGISHIMA Motohiro
Research Laboratories, Diagnostic Division, Toyo Jozo Co., Ltd.
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NAKAMURA Shoshiro
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
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IMAMURA Shigeyuki
Research Laboratories, Diagnostic Division, Toyo Jozo Co., Ltd.
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IMAMURA Shigeyuki
Central Laboratory, Asahi Chemical Industry Co., Ltd.
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Inouye Y
Hiroshima Univ. School Of Medicine Hiroshima Jpn
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Sugiyama Masanori
Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
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Suzuki K
Department Of Biotechnology Tottori University
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Inouye Yoshio
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Nakamura Shoshiro
Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
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Imamura S
Asahi Chemical Ind. Co. Ltd. Shizuoka
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Suzuki Koji
Research Laboratories Of Brewing Technology Asahi Breweries Ltd.
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Ogishima Motohiro
Research Laboratories Diagnostic Division Toyo Jozo Co. Ltd.
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Suzuki Koji
Research Institute Of Instrumentation Frontier National Institute Of Advanced Industrial Science And
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