Identification of a Penicillin-Sensitive Carboxypeptidase in the Cellular Slime Mold Dictyostelium discoideum
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概要
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Penicillin binding proteins (PBPs) are penicillin-sensitive DD-peptidases catalyzing the terminal stages of bacterial cell wall assembly. We identified a Dictyostelium discoideum gene that encodes a protein of 522 amino acids showing similarity to Escherichia coli PBP4. The D. discoideum protein conserves three consensus sequences (SXXK, SXN and KTG) that are responsible for the catalytic activities of PBPs. The gene product prepared in the cell-free translation system showed carboxypeptidase activity but the activity was not detected in the presence of penicillin G. These results demonstrate that the D. penicillin-sensitive carboxypeptidase.
- 2003-07-01
著者
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YAMAGUCHI Kazuo
Institute for Gene Research, Kanazawa University
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KUROITA TOSHIHIRO
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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Tamura Kentaro
Division Of Bio Engineering Faculty Of Engineering Toyama University
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YASUKAWA HIRO
Division of Bioengineering, Faculty of Engineering, Toyama University
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Kuroita Toshihiro
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd
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Kuroita Toshihiro
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Yasukawa Hiro
Division Of Bio Engineering Faculty Of Engineering Toyama University
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Yamaguchi Kazuo
Institute For Gene Research Kanazawa University
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Yamaguchi Kazuo
Institue Of Interdisciplinary Research Faculty Of Engineering University Of Tokyo
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