Semi-batch Culture of Microorganisms with Constant Feed of Substrate : An Experimental Study : Kinetic Studies on Fed-batch Cultures (V)
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概要
- 論文の詳細を見る
Semi-batch cultures of Enterobacter cloacae NCIB 8271 with constant feed of glycerol or of its aqueous solution were carried out in order to verify the inequality and the approximate equations proposed in the authors' previous paper (J. Ferment. Technol., 55,156 (1977)).The increment in the culture volume arising from the glycerol-feeding was determined to be 0.64 ml of culture broth/ml of glycerol fed, implying that the volume increase, if any, came mainly from the water content in the feed solution. The maintenance coefficient of the bacterium with respect to glycerol was negligible. The cell yield in the linear growth phase was the same as that in the exponential growth phase.All data of time courses of cell concentration both in the exponential growth phase and in the linear growth phase and of glycerol concentration in the exponential growth phase agreed well with the approximate equations proposed previously by the authors. The glycerol concentraiton in the linear growth phase was too small to determine accurately by the adopted assay method.Transition time from the exponential growth phase to the linear growth phase could be well predicted by the equation of the transition time proposed previously by the authors.Continuous monitoring of the dissolved oxygen concentration was very useful not only for starting up the glycerol-feeding when the substrate concentration in the culture liquid was nearly zero but also for discerning the transition time indirectly in the course of fed-batch cultivation.
- 社団法人日本生物工学会の論文
- 1977-08-25
著者
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YAMANE Tsuneo
Department of Biological Mechanisms and Functions, Graduate School of Bio- and Agro-Sciences, Nagoya
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Hirano Shigeki
(present Address)research Center Osaka Gas Co.
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Yamane Tsuneo
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
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