Highly accumulative production of L(+)-lactate from glucose by crystallization fermentation with immobilized Rhizopus oryzae(BIOCHEMICAL ENGINEERING)
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概要
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In order to produce microbiologically large amount of L(+)-lactic acid (LA) from glucose, batch and fed-batch (intermittent addition of sterillzed glucose powder aseptically) cultures of Rhizopus oryzae NBRC 5384 (identical to NRRL 395 and ATCC 9363) whose mycelia were immobilized in situ within sponge-like cubic particles (3.5 mm edge long) were carried out at 37℃ in a three baffled shake flask. Appropriately calculated amount of fine powdery calcium carbonate (CaCO_3) was added initially or intermittently to control pH of the culture liquids. High accumulations of LA (145 g/L and 231 g/L, in reality 176 g/L and 280 g/L as anhydrous calcium lactate) were achieved by a batch (glucose concentration = 150 g/L) and a fed-batch cultures (the initial glucose concentration = 150 g/L and the intermittent addition of glucose equivalent to 100 g/L). In these cultures the yields and productivities of LA were, 95.0%, 1.42 g/L・h and 92.5%, 1.83 g/L・h, respectively. Existence of considerable amounts of calcium lactate (Ca(LA)_2) as crystals in the culture broth was experimentally proved by two evidences: (i) heating up (70℃) followed by quick low centrifugal force to remove remaining CaCO_3 solids from culture broth and then cooling down (37℃) followed by incubation of the culture supernatant at 37℃ to observe recrystallization of Ca(LA)_2, and (ii) the measurement of solubility of Ca(LA)_2 in the culture media. It was conceptually discussed to be able to avoid the product inhibition by means of crystallization fermentation for the high accumulation of LA by R oryzae.
- 2013-01-00
著者
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Yamane Tsuneo
Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University
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Yamane Tsuneo
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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Tanaka Ryohsuke
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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