Signal Peptidase Cleavage Site in the Processing of Pseudomonas aeruhginosa Preproelastase
スポンサーリンク
概要
- 論文の詳細を見る
The extracellular elastase (33 kDa) of Pseudomonas aeruginosa is synthesized as a 53.6-kDa preproenzyme containing a long N-terminal propeptide, which is processed to the mature form via a 51-kDa proelastase. A 51-kDa protein isolated from Escherichia coli transformant carrying the Glu^<141>→Gln mutant elastase gene was subjected to N-terminal amino acid sequence analysis. No autoproteolytic processing of proelastase was expected to occur in these cells. The data indicated that the N-terminal sequence corresponds to the position between -174 and -164 of the preproelastase (numbers are in reference to the first amino acid residue of mature elastase). This confirms that the 51-kDa protein is proelastase and that the signal peptidase cleaves between Ala^<-175> and Ala^<-174>.
- 社団法人日本生物工学会の論文
- 1994-10-25
著者
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Inagaki Kenji
Division of Bioresources Science, Faculty of Agriculture, Okayama University
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OKUDA Kenji
Department of Bacteriology, Yokohama City University School of Medicine
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KAWAMOTO Susumu
Department of Bacteriology, Yokohama City University School of Medicine
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Okuda K
Kyushu Univ. Fukuoka Jpn
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Kawamoto Susumu
Department Of Bacteriology Yokohama City University School Of Medicine
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Shibano Yuji
Suntory Research Center:(present Address)suntory Ltd.
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Shibano Yuji
Department Of Molecular Biology Biomolecular Engineering Research Institute
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Shibano Yuji
Department Of Moleular Biology Biomolecular Engineering Research Insitute
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Shibano Yuji
Institute For Fundamental Research Suntory Ltd.
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Shibano Yuji
Institute For Fundamental Research Suntory Limited
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Fukushima J
Department Of Biotechnology Faculty Of Bioresource Sciences Akita Prefectural University
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Okuda Kenji
Department Of Bacteriology Yokohama City University School Of Medicine
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FUKUSHIMA Jun
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University
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MORIHARA Kazuyuki
Institute for Applied Life Science, University of East Asia, Graduate School
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Inagaki Kenji
Division Of Bioresources Science Faculty Of Agriculture Okayama University
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Morihara K
Institute For Applied Life Science University Of East Asia Graduate School
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Morihara Kazuyuki
Institute For Applied Life Science Graduate School University Of East Asia
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Yamashita Satoshi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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FUKUSHIMA Jun
Department of Applied Chemistry, Tohoku University
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FUKUSHIMA Jun
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University
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