Site-Directed Mutagenesis of His-176 and Glu-177 in Pseudomonas aeruginosa Alkaline Protease : Effect on Catalytic Activity

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概要

• 論文の詳細を見る

• It has been hypothesized that H-176 and E-177 are key residues in the catalytic activity of Pseudomonas aeruginosa alkaline protease. To test this possilbilty, we replaced H-176 with L-leucine and E-177 with L-glutamine using site-directed mutagenesis. The mutant enzyme proteins, produced by the Escherichia coli transformants harboring mutated genes, did not show any proteolytic activity without alteration of their molecular sizes compared to the wild-type enzyme protein.

• 社団法人日本生物工学会の論文
• 1997-12-25

著者

• OKUDA Kenji

  Department of Bacteriology, Yokohama City University School of Medicine

• KAWAMOTO Susumu

  Department of Bacteriology, Yokohama City University School of Medicine

• SHIBANO YUJI

  Department of Nutritional Physiology, Faculty of Nutrition, Kobe Gakuin University

• 畑 安雄

  京大・化研

• Okuda K

  Kyushu Univ. Fukuoka Jpn

• HATA Yasuo

  Institute for Chemical Research, Kyoto University

• 畑 安雄

  Institute For Chemical Research Kyoto University

• Kawamoto Susumu

  Department Of Bacteriology Yokohama City University School Of Medicine

• Hata Yasuo

  Institute For Chemical Research Kyoto University

• Shibano Yuji

  Suntory Research Center:(present Address)suntory Ltd.

• Shibano Yuji

  Department Of Molecular Biology Biomolecular Engineering Research Institute

• Shibano Yuji

  Department Of Moleular Biology Biomolecular Engineering Research Insitute

• Fukushima J

  Department Of Biotechnology Faculty Of Bioresource Sciences Akita Prefectural University

• Okuda Kenji

  Department Of Bacteriology Yokohama City University School Of Medicine

• FUKUSHIMA Jun

  Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University

• MORIHARA Kazuyuki

  Institute for Applied Life Science, University of East Asia, Graduate School

• MIYAJIMA Yoshiro

  Institute for Applied Life Science, Graduate School of University of East Asia

• Morihara K

  Institute For Applied Life Science University Of East Asia Graduate School

• Morihara Kazuyuki

  Institute For Applied Life Science Graduate School University Of East Asia

• Miyajima Y

  Institute For Applied Life Science Graduate School Of University Of East Asia

• Miyajima Yoshiro

  Institute For Applied Life Science Graduate School University Of East Asia

• Yamashita Satoshi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

• FUKUSHIMA Jun

  Department of Applied Chemistry, Tohoku University

• FUKUSHIMA Jun

  Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University

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