Use of Bacillus brevis for Synthesis and Secretion of Des-B30 Single-Chain Human Insulin Precursor
スポンサーリンク
概要
- 論文の詳細を見る
A synthetic gene encoding a single chain human insulin precursor [B-chain (1-29)-A-chain] linked to the Cterminal lysine of human epidermal growth factor (1-28) (EGF-SCI) was constructed. This gene was expressed using Bacillus brevis. EGF-SCI was isolated from the supernatant of the culture broth. Treatment of EGF-SCI with lysyl endopeptidase resulted in the formation of des-B30 human insulin. The identification of the formed des-B30 human insulin was made by the measurement of molecular weight and amino acid analysis. The binding coefficient to anti-human insulin antibody was comparable to that of human insulin.
- 社団法人日本農芸化学会の論文
- 2000-05-23
著者
-
Ebisu Shogo
R & D Department Higeta Shoyu Co. Ltd.
-
Takagi Hiroaki
R & D Department Higeta Shoyu Co. Ltd.
-
TAKAGI Hiroaki
R & D Department, Higeta Shoyu Co., Ltd.
-
Hanagata Hiroshi
R & D Department Higeta Shoyu Co. Ltd.
-
KOH Mamoru
Institute for Applied Life Science, University of East Asia, Graduate School
-
MORIHARA Kazuyuki
Institute for Applied Life Science, University of East Asia, Graduate School
-
Koh Mamoru
Institute For Applied Life Science University Of East Asia Graduate School
-
Morihara Kazuyuki
Institute For Applied Life Science Graduate School University Of East Asia
-
Morihara Kazuyuki
Institute For Applied Life Science University Of East Asia Graduate School
-
EBISU Shogo
R & D Department, Higeta Shoyu Co., Ltd.
-
HANAGATA Hiroshi
R & D Department, Higeta Shoyu Co. Ltd.
関連論文
- Antitumor Activity of Brasilicardin A, a Novel Terpenoid Antibiotic from Nocardia brasiliensis
- Distribution of Sarcophytol A in Soft Coral of the Sarcophyton Genus
- Signal Peptidase Cleavage Site in the Processing of Pseudomonas aeruhginosa Preproelastase
- Long-Range Effect of Mutation of Calcium Binding Asparates on the Catalytic Activity of Alkaline Protease from Pseudomonas aeruginosa^1
- Site-Directed Mutagenesis of His-176 and Glu-177 in Pseudomonas aeruginosa Alkaline Protease : Effect on Catalytic Activity
- Structural Conversion from Non-native to Native form of Recombinant Human Epidermal Growth Factor by Brevibacillus choshinensis
- Use of Bacillus brevis for Synthesis and Secretion of Des-B30 Single-Chain Human Insulin Precursor
- Crystal Structure of the Unliganded Alkaline Protease from Pseudomonas aeruginosa IFO3080 and Its Conformantional Changes on Ligand Binding
- Papain-Catalyzed Synthesis of Aspartame Precursor : A Comparison with Thermolysin