Isolation of Microorganisms That Lyse Filamentous Bacteria and Characterization of the Lytic Substance Secreted by Bacillus polymyxa
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概要
- 論文の詳細を見る
Microorganisms capable of lysing filamentous bacteria were isolated from activated sludge by screening for their ability to form plaques on an agar plate seeded with cells of Sphaerotilus natans. The supernatant obtained by culturing of these microorganisms lysed filamentous bacteria in a liquid culture. After purification using column chromatography followed by anion-exchange, gel-filtration and hydroxyapatite chromatography, the lytic substance produced by of B. polymyxa showed the highest lytic activity in all of screened bacteria. However, a specific band representing the substance responsible for the lytic activity could not be observed on SDS-polyacrylamide gel. In addition, the lytic substance could not be rendered inactive by various enzyme denaturing treatments. The substance has properties similar to those of a biosurfactant that is often produced by B. subtilis.
- 公益社団法人日本生物工学会の論文
- 1996-10-25
著者
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Fukase Tetsuro
Kurita Central Laboratories Kurita Water Industries Ltd.
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Miyata Hiroshi
Kurita Central Laboratories Kurita Water Industries Ltd.
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KITATSUJI KATSURA
Kurita Central Laboratories, Kurita Water Industries Ltd.,
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Kitatsuji Katsura
Kurita Central Laboratories Kurita Water Industries Ltd.
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Fukase Tetsuro
Kurita Central Laboratories, Kurita Water Industries Ltd.,
関連論文
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- Purification and Characterization of a Thermostable Lipase from Newly Isolated Pseudomonas sp. KWI-56(Microbiology & Fermentation Industry)
- Role of the Gene Encoding Lipase Activator from Pseudomonas sp. Strain KWI-56 in in Vitro Activation of Lipase
- Isolation of Microorganisms That Lyse Filamentous Bacteria and Characterization of the Lytic Substance Secreted by Bacillus polymyxa