Reaction Kinetics and Modeling of the Enzyme-catalyzed Production of Lactosucrose using β-Fructofuranosidase from Arthrobacter sp. K-1

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概要

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• Lactosucrose synthesis from sucrose and lactose was carried out by using β-fructofuranosidase from Arthrobacter sp. K-1. The transfructosylation mechanism was found to be of an ordered bi-bi type in which sucrose was bound first to the enzyme and lactosucrose was released last. Hydrolysis side-reaction experiments indicated that the reactions were uncompetitively inhibited by glucose and lactose, while no inhibition by fructose was apparent. The overall reaction rates were formulated. The reaction rate constants, equilibrium constant, and dissociation and Michaelis constants were determined at 35℃ and 50℃ by fitting the experimental concentration changes with the calculated values by a nonlinear least-square method. The average relative derivation for the concentrations was 9.67%. The kinetic parameters were also calculated for 43℃ and 60℃ by assuming the Arrhenius law, and the course of reaction was predicted. The obtained reaction rate equations well represented the concentration changes during the experiment at all temperatures.

• 社団法人日本農芸化学会の論文
• 2001-04-23

著者

• HASHIMOTO Kenji

  Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine

• Fujita Koki

  Bio Research Corporation Of Yokohama

• KAWASE Motoaki

  Department of Chemical Engineering, Faculty of Engineering, Kyoto University

• Kawase M

  Kyoto Univ. Kyoto

• Kawase Motoaki

  Department Of Chemical Engineering Faculty Of Engineering Kyoto University

• Pilgrim Axel

  Department Of Chemical Engineering Faculty Of Engineering Kyoto University

• OHASHI Masayasu

  Department of Chemical Engineering, Faculty of Engineering, Kyoto University

• MURAKAMI Kazufumi

  Bio Research Corporation of Yokohama

• Ohashi Masayasu

  Department Of Chemical Engineering Faculty Of Engineering Kyoto University

• Hashimoto Kenji

  Department Of Chemical Engineering Faculty Of Engineering Kyoto University

• Hashimoto Kenji

  Department Of Anatomy Nara Medical University

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