Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice
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概要
- 論文の詳細を見る
In conjunction with Ca2+ signaling, the subcellular localization of Ca2+ channels in various plant cells has been under intense scrutiny. The present study focused on the subcellular localization of OsTPC1, a putative voltage-dependent calcium channel in rice. A newly developed antibody to the linker domain of OsTPC1 was shown to bind this protein in vitro. Immunoblotting of a plasma membrane (PM)-enriched fraction obtained by aqueous two-phase partitioning, as well as indirect immunofluorescence confocal microscopy of rice protoplasts, supported the idea that OsTPC1 is predominantly localizes in the PM, at least of suspension-cultured rice cells.
- 日本植物細胞分子生物学会の論文
著者
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HASHIMOTO Kenji
Department of Oral and Maxillofacial Surgery, Hamamatsu University School of Medicine
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SAITO MIKAKO
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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MATSUOKA HIDEAKI
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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IIDA Hidetoshi
Department of Biology, Tokyo Gakugei University
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