Separation of Viable Histamine-Producing Bacteria from Yellowtail Meat Components by Density Gradient Centrifugation
スポンサーリンク
概要
- 論文の詳細を見る
Quantitative separation of live cells from food samples is essential for non-culture methods to be validated. In this viewpoint, the feasibility of density gradient centrifugation (DGC) was demonstrated for the first time using samples of yellowtail meat to which Morganella morganii, a histamine producing bacterium had been added. Using a Ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of M. morganii cells were collected in 20-50 w/v % layers. The total cell collection rate ranged from 73-86 % irrespective of the cell density in the range 102-106 cells/200 µl.
- 日本防菌防黴学会の論文
- 2009-03-10
著者
-
SAITO MIKAKO
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
MATSUOKA HIDEAKI
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
-
Sasaki Mami
Plant Science Center Riken
-
Saito Mikako
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Araki Emiko
Japan Food Research Laboratory
-
Matsuoka Hideaki
Department Of Biotechnology And Life Science Faculty Of Technology Tokyo University Of Agriculture A
-
Matsuoka Hideaki
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Araki Emiko
Japan Food Research Laboratories
-
TAKAYAMA KODAI
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
-
Takayama Kodai
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
-
Saito Mikako
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
関連論文
- A Comprehensive Gene Expression Analysis Toward the Understanding of Growth and Differentiation of Tobacco BY-2 Cells
- Cytotoxicity of Extracts from Alternaria alternata against Cultured Tobacco BY-2 Cells
- Distinctive Effects of Menadione on the In Vivo Catalase Activity and the Adaptation to Hydrogen Peroxide Stress in Yeast Cells
- Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells
- Cell-lytic Activity of Tobacco BY-2 Induced by a Fungal Elicitor from Alternaria alternata Attributed to the Expression of a Class I β-1, 3-Glucanase Gene
- Isolation of a Novel Isozyme of Tobacco BY-2 Chitinase Induced by a Fungal Elicitor
- Delection of Antifungal Activity in Belamcanda chinensis by a Single-cell Bioassay Method and Isolation of Its Active Compound, Tectorigenin
- Upstream-directed Growth of Filamentous Fungi in a Flowing Medium
- タバコ培養細胞細胞間膜への定量的傷害印加のための微小電極システムの応用
- Simultaneous Imaging of Multiple Fluorescent Probes in Bio-Cells
- Vitamin B12 Promotes Cx40 and HCN4 Gene Expression at an Early Stage of Cardiomyocyte Differentiation
- ＜Poster＞Synthesis of 2-NBDLG, the antipode of fluorescent D-glucose tracer 2-NBDG
- ＜Symposium I＞A new imaging method of glucose transport in live cells
- Sensitive Detection of Antifungal Activity of Volatile Components Emitted from Plant Leaf Samples by Single Hypha Assay Method
- Evaluation of Antifungal Activity of an Antifungal Drug by In Vitro Simulation of In Vivo Pharmacokinetics of the Drug against Fungal Hyphal Growth
- Rapid and Sensitive Screening of Antifungal Activity in Medicinal Plants by a Single-cell Biosensing System
- CONTRACTIONS AND SLOW ACTION POTENTIALS OF ISCHEMIC AND REPERFUSED HEART MUSCLE : PROTECTIVE EFFECT OF COENZYME Q_ : Cardiovascular drugs (II) : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- 電気的シグナルによるイネ単一細胞内へのCa〔2+〕流入の制御
- Automatic Mapping of Viable Microbial Cells Distributed in the Surface Layer of Cotton Fabrics
- Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine(Food & Nutrition Sience)
- タバコ培養細胞における膜電位制御と膜インピーダンスの同時計測
- Functional Analysis of a Rice Putative Voltage-Dependent Ca^ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1
- Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice
- Single-Cell Imaging of the Ca^2+ Influx into Bovine Endothelial Cells Occurring in Response to an Alternating Electric
- Comprehensive Analysis of Glucan Elicitor-Regulated Gene Expression in Tobacco BY-2 Cells Reveals a Novel MYB Transcription Factor Involved in the Regulation of Phenylpropanoid Metabolism
- Evaluation of a New Most-Probable-Number (MPN) Dilution Plate Method for the Enumeration of Escherichia coli in Water Samples
- Influence of antifungal pre-treatment in preparing test mycelium on MIC values of several antifungal agents against Aspergillus niger
- Production of a Cloned Mouse by Nuclear Transfer from a Fetal Fibroblast Cell of a Mouse Closed Colony Strain
- Isolation and Properties of an Extracellular β-Glucosidase from a Filamentous Fungus, Cladosporium resinae, Isolated from Kerosene
- Development of a Density Slicer for the Simple Collection of Respective Density Layers after Stepwise Density Gradient Centrifugation
- Separation of Viable Histamine-Producing Bacteria from Yellowtail Meat Components by Density Gradient Centrifugation
- Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different
- Evidence for the plasma membrane localization of a putative voltage-dependent Ca^ channel, OsTPC1, in rice
- 単一細胞実験法のためのマイクロバイオエレクトロニクス
- 単一細胞バイオエレクトロケミストリーを目指す高効率マイクロインジェクション技術
- ES細胞分化のFACS解析における蛍光グルコース2-NBDGの代謝活性指標としての利用
- Analysis of Odor Compounds in Feces of Mice that Were Exposed to Various Stresses during Breeding
- Development of a Protocol for Selection of GenesFit for the In Vivo Knockdown Method and its Application to Insulin Receptor Substrate Genesin Mice
- Potential Use of Lactobacillus Cell Density in Feces as a Non-invasive Bio-indicator for Evaluating Environmental Stress During Mouse Breeding
- Automatic Stop of a Microinjector Distinctively in the Cytosol or the Vacuole of Plant Single-cells
- Electric Gene Expression in Single-cells of Rice Protoplast via Ca2+ Entering the Cell
- Development of a Protocol for Selection of Genes Fit for the In Vivo Knockdown Method and its Application to Insulin Receptor Substrate Genes in Mice
- Analysis of Odor Compounds in Feces of Mice that Were Exposed to Various Stresses during Breeding