ES細胞分化のFACS解析における蛍光グルコース2-NBDGの代謝活性指標としての利用
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概要
- 論文の詳細を見る
The feasibility of using the fluorescent glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) as a novel tool for FACS analysis during the ES cell differentiation process has been discussed. 2-NBDG entered into the ES cells via a D-glucose specific uptake activity and a general metabolic activity. Although the 2-NBDG uptake was not distinct enough for discriminating cell types, changes in the histogram of 2-NBDG loaded cells during differentiation paralleled with their morphological changes. Thus, it was suggested that 2-NBDG could be used for monitoring the metabolic status of cells during differentiation as an additional marker for higher resolution analysis by FACS.
著者
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Saito Mikako
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Matsuoka Hideaki
Department Of Biotechnology And Life Science Faculty Of Technology Tokyo University Of Agriculture A
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FUNABASHI Hisakage
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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OGINO Shiho
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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