Vitamin B12 Promotes Cx40 and HCN4 Gene Expression at an Early Stage of Cardiomyocyte Differentiation
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概要
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Vitamin B12 (VB12) was investigated for its ability to influence the expression of genes required for cardiac cell differentiation from mouse embryonic stem cells. When VB12 (0.5 mM) was added to the medium on day 3 of culture, the levels of Cx40 and HCN4 expression increased over those in the control, 2 to 3 days before the start of cardiomyocyte beating. In contrast, the expression levels of α-MHC, MLC-2a, and MLC-2v were almost the same as those of the control throughout the culture period. Our results suggest that VB12 is involved in the promotion of the intercellular conducting system rather than the generation of contractile proteins.
著者
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Saito Mikako
Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology
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Matsuoka Hideaki
Department Of Biotechnology And Life Science Faculty Of Technology Tokyo University Of Agriculture A
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SASAKI Toshiya
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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