Automatic Stop of a Microinjector Distinctively in the Cytosol or the Vacuole of Plant Single-cells

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概要

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• A 3-channel (3C) microinjector was prepared by pulling a glass capillary. One channel was used for a potential measuring electrode (MeaE) and the other two channels were used for electrophoretic introduction electrode (IntE) and its counter electrode (CE). The 3C microinjector was propelled by an oil pressure manipulator that was driven by a pulse motor, while the MeaE output was recorded continuously. When the 3C microinjector penetrated the cell membrane of a tobacco cultured cell, BY-2, the output potential changed sharply towards the negative direction. Then the microinjector progressed further into the cell. A marked change of the output potential occurred again, though it was towards the positive direction. These changes were speculated as the passages of cell membrane and vacuolar membrane, respectively. Using the differential of these changes as the stop signal, the microinjector could rightly be stopped either in the cytosol or the vacuole, which was confirmed by the dye diffusion pattern.

• 公益社団法人 電気化学会の論文

著者

• Saito Mikako

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology

• Miwa Yoshiyuki

  Faculty Of Mechanical Engineering Waseda University

• Matsuoka Hideaki

  Department Of Biotechnology And Life Science Faculty Of Technology Tokyo University Of Agriculture A

• Horikiri Shigetoshi

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology

• YAMADA Yohei

  CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency

• HATTA Keisuke

  Faculty of Science and Engineering, Waseda University

• KUBO Tomoaki

  Faculty of Science and Engineering, Waseda University

• KUSHIHASHI Yasuhiro

  Department of Computer, Information and Communication Sciences

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