Reverse Reaction of Aspergillus niger APC-9319 α-Galactosidase in a Supersaturated Substrate Solution : Production of α-Linked Galactooligosaccharide (α-GOS)
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概要
- 論文の詳細を見る
The α-galactosidase that effectively catalyzes a reverse reaction of galactose, Aspergillus niger APC-9319 α-galactosidase, was screened from industrial enzyme preparations for food processing containing α-galactosidase activity. Reverse reaction of A. niger APC-9319 α-galactosidase was performed using a supersaturated solution (90% galactose [w/v]). A. niger APC-9319 α-galactosidase was not inhibited even in high substrate concentration, and effectively catalyzed the reverse reaction. The yield of the reaction product, α-linked galactooligosaccharide (α-GOS), increased greatly as the initial concentration of galactose increased to 90% (w/v), and was more than 50%. Furthermore, the half life of enzyme activity was about three times as long as that using 60% galactose (w/v). α-GOS (1.4 g) was prepared from galactose (3.0 g) by reverse reaction of A. niger APC-9319 α-galactosidase. The α-GOS contained 58% α-galactobiose (α-Gal2), 28% α-galactotriose, and 14% oligosaccharides larger than α-galactotriose. The main component of positional isomers in α-Gal2 was α-1,6Gal2.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
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YAMASHITA AKIKO
Department of Anesthesiology, National Cardiovascular Center
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Fujita Koki
北海道大学 農学研究院応用生命科学
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Fujita Koki
Bio Research Corporation Of Yokohama
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HASHIMOTO Hiroyuki
Department of Applied Biology, Kyoto Institute of Technology
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Yamashita Akiko
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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KITAHATA Sumio
Department of Bioscience and Biotecnology, Shinshu University
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Mori Shigeharu
Amano Pharmaceutical Co., Ltd.
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Mori Shigeharu
Amano Enzyme
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Kitahata Sumio
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Okada Masamichi
Amano Enzyme
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Hashimoto Hiroyuki
Department Of Agricultural Chemistry Kochi University
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Hashimoto Hiroyuki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Fujita Koki
Carbohydrate Research Laboratory Ensuiko Sugar Refining Co. Ltd.
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Mori Shigeharu
Amano Enzyme Inc.
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Yamashita Akiko
Department Of Anatomy Nihon University School Of Medicine
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