Isolation and Characterization of a β-Primeverosidase-Like Enzyme from Penicillium multicolor
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概要
- 論文の詳細を見る
p-Nitrophenyl and eugenyl β-primeveroside (6-O-β-D-xylopyranosyl-β-D-glucopyranoside) hydrolytic activity was found in culture filtrate from Penicillium multicolor IAM7153, and the enzyme was isolated. The enzyme was purified as a β-primeverosidase-like enzyme by precipitation with ammonium sulfate followed by successive chromatographies on Phenyl Sepharose, Mono Q, and β-galactosylamidine affinity columns. The molecular mass was estimated to be 50 kDa by SDS–PAGE and gel filtration. The purified enzyme was highly specific toward the substrate p-nitrophenyl β-primeveroside, which was cleaved in an endo-manner into primeverose and p-nitrophenol, but a series of β-primeveroside as aroma precursors were hydrolyzed only slightly as substrates for the enzyme. In analyses of its hydrolytic action and kinetics, the enzyme showed narrow substrate specificity with respect to the aglycon and glycon moieties of the diglycoside. We conclude that the present enzyme is a kind of β-diglycosidase rather than β-primeverosidase.
- 社団法人 日本農芸化学会の論文
- 2006-03-23
著者
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村田 健臣
静岡大学創造科学技術大学院
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Sakata Kanzo
Institute for Chemical Research, Kyoto University
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Usui Taichi
Science Of Biological Resource The United Graduate School Of Agricultural Science Gifu University
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Murata Takeomi
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Murata Takeomi
Department Of Applied Biological Cemistry Faculty Of Agriculture Shizuoka University
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Murata Takeomi
Department Of Biological Chemistry Faculty Of Agriculture Shizuoka University
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Usui Takeo
Department Of Biotechnology Faculty Of Agriculture And Life Science University Of Tokyo
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Mori Shigeharu
Amano Pharmaceutical Co., Ltd.
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Murata T
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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AMARUME Satoshi
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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TSURUHAMI Kazutaka
Science of Biological Resources, The United Graduate School of Agricultural Science, Gifu University
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SARUWATARI Shigetaka
Department of Biological Chemistry, Faculty of Agriculture, Shizuoka University
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HIRAKAKE Jun
Institute for Chemical Research, Kyoto University
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Hirakake Jun
Institute For Chemical Research Kyoto University
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Usui T
Dep. Of Applied Biological Chemistry Fac. Of Agriculture Shizuoka Univ.
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Sakata Kanzo
Institute For Chemical Research Kyoto University
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Mori Shigeharu
Amano Enzyme
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Mori Shigeharu
Amano Enzyme Inc. Gifu R&d Center
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Amarume Satoshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tsuruhami Kazutaka
Science Of Biological Resources The United Graduate School Of Agricultural Science Gifu University
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Saruwatari Shigetaka
Department Of Biological Chemistry Faculty Of Agriculture Shizuoka University
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Usui Taichi
Department Of Bioscience Graduate School Of Science And Technology Shizuoka University
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