A Combined Assay of Cell Vability and in Vitro Cytotoxicity with a Highly Water-Soluble Tetrazolium Salt, Neutral Red and Crystal Violet
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概要
- 論文の詳細を見る
Cell viability and in vitro cytotoxicity assay methods were developed using a combination of dyes, 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), neutral red (NR) and crystal violet (CV), with HeLa cells as a bioindicator. As WST-1 produces a highly water soluble and non-cytotoxic formazan dye, it allows each assay to be carried out in one culture dish. The combined cell viability assay using WST-1,NR and CV gave an absorbance that correlated linearly with the number of cells over the range 1000 to 50000 cells/well. The combined assay was applied to the evaluation of the IC_<50> values for sodium dodecyl sulfate as a model toxicant, which yielded similar values to those obtained with each assay independently.
- 公益社団法人日本薬学会の論文
- 1996-11-15
著者
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Ueno K
Department Of Pharmaceutical Sciences Niigata University Of Pharmacy And Applied Life Sciences
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Ueno Kazuyuki
国立医薬品食品衛生研究所 薬物応答予測プロジェクト
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Ueno Kazuyuki
新潟薬科大学 薬剤学
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Ohkura Y
Kyushu Univ. Fukuoka Jpn
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Tominaga Hideyuki
Dojido Laboratories
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Ueno Koichi
Departments Of Geriatric Pharmacology And Therapeutics Graduate School Of Pharmaceutical Sciences Ch
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Shiga Masanobu
Dojindo Laboratores
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Sasamoto Kazumi
Dojindo Laboratores
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ISHIYAMA Munetaka
Dojido Laboratories
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SHIGA Masanobu
Dojido Laboratories
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SASAMOTO Kazumi
Dojido Laboratories
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OHKURA Yosuke
Dojido Laboratories
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UENO Keiyu
Dojido Laboratories
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Ishiyama M
Dojindo Laboratores
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Urashima Kazuya
Department Of Pharmaceutical Sciences Niigata University Of Pharmacy And Applied Life Sciences
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