Characteristics of Chymotrypsin Modified with Water-soluble Acylating Reagents and Its Peptide Synthesis Ability in Aqueous Organic Media
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概要
- 論文の詳細を見る
Several kinds of modified chymotrypsin were prepared with water-soluble acylating reagents, and their characteristics after hydrolyzing with unmodified chymotrypsin in aqueous-N, N'-dimethylformamide (DMF) media were compared. It was found that chymotrypsin (Csin), of which a 20% amino group was modified with a benzyloxycarbonyl group (Z(20)Csin), had more favorable characteristics than unmodified chymotrypsin with regard to hydrolytic activity in an aqueous DMF media. We also investigated the Z(20)Csin-catalyzed peptide synthesis in two different solution systems. In the one-layer system containing water and DMF, Z(20)Csin catalyzed the peptide bond formation in a higher yield than that by unmodifide chymotrypsin and enabled a synthetic reaction in even an 80% (v/v) DMF media, in which the hydrolytic reaction could not be carried out. Z(20)Csin catalyzed the condensation between some N-acyl amino acids or peptide derivatives and amino acids in 90% ethylacetate, 90% hexane or 50% benzene. This latter method employs a two-layer system, and the modified enzyme may be able to reduce the number of synthetic steps when preparing acyl peptides.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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NAKAMURA Tsutomu
Department of Hospital Pharmacy, Kobe University School of Medicine
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KAWASAKI Yoshihiro
Technology and Research Institute, Snow Brand Milk Products Co., Ltd.
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OKAI Hideo
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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KAWASAKI Yoshihiro
Fujitsu Laboratories Ltd., Wireless Communication Systems Laboratories
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Kawasaki Yoshihiro
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Kawasaki Yoshihiro
Fujitsu Laboratories Ltd. Wireless Communication Systems Laboratories
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Kawasaki Yoshihiro
Technical Research Institute Snow Brand Milk Products Co.
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Kawasaki Y
Snow Brand Milk Products Co. Ltd. Saitama Jpn
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Nakamura Tsutomu
Department Of Gastroenterological Surgery Kobe University Graduate School Of Medical Sciences
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Murakami Mototake
Technical Research Institute, Snow Brand Milk Products Co., Ltd.
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Okai H
Hiroshima Univ. Hiroshima Jpn
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Okai Hideo
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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AZUSE Ikunori
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Murakami Mototake
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Azuse Ikunori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nakamura Tsutomu
Department Of Chemistry Nara Womens University
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Dosako S
Snow Brand Co. Ltd. Saitama Jpn
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Nakamura Tsutomu
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nakamura Tsutomu
Department Of Chemistry Faculty Of Science Faculty Of Agriculture Kyoto Unviersity
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