Stirred Culture of Myeloma MPC-11 Cells in Serum-free Medium
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概要
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On addition of soy bean phospholipid fraction to serum-free medium (TES medium), immunoglobulin producing myeloma MPC-11 cells could grow under stirring in the serum-free mediumalmost as well as in serum medium. This is the first success in culturing cells in stirred serum-free conditions. Technologically important parameters such as temperature, stirring velocity and aeration of the mediumwere also optimized in the serum-free stirred culture. These results will provide somefundamental knowledge for developing a large-scale suspension culture system especially for immunoglobulin producing cells.
著者
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Omura Hirohisa
Department Of Food And Nutrition Nakamura-gakuen College
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SHINOHARA Kazuki
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Murakami Hiroki
Department of Analytical and Bioinorganic Chemistry, Division of Analytical & Physical Sciences, Kyoto Pharmaceutical University
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EDAMOTO Toshikazu
Department of Food Science & Technology, Faculty of Agriculture, Kyushu University
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MURAKAMI Hiroki
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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OMURA Hirohisa
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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EDAMOTO Toshikazu
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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SHINOHARA Kazuki
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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SHINOHARA Kazuki
Department of Food Science and Technology, Faculty of Agriculture
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