Cloning of the Maltose Phosphorylase Gene from Bacillus sp. Strain RK-1 and Efficient Production of the Cloned Gene and the Trehalose Phosphorylase Gene from Bacillus stearothermophilus SK-1 in Bacillus subtilis(Biochemistry & Molecular Biology)
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概要
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The maltose phosphorylase (MPase) gene of Bacillus sp. strain RK-1 was cloned by PCR with oligonucleotide primers designed on the basis of a partial N-terminal amino acid sequence of the purified enzyme. The MPase gene consisted of 2,655 bp encoding a theoretical protein with a M_r of 88,460, and had no secretion signal sequence, although most of the MPase activity was detected in the culture supernatant of RK-1. This cloned MPase gene and the trehalose phosphorylase (TPase) gene from Bacillus stearothermophilus SK-1 were efficiently expressed intracellularly under the control of the Bacillus amyloliquefaciens α-amylase promoter in Bacillus subtilis. The production yields were estimated to be more than 2 g of enzyme per liter of medium, about 250 times the production of the original strains, in a simple shake flask. About 60% of maltose was converted into trehalose by the simultaneous action of both enzymes produced in B. subtilis.
- 社団法人日本農芸化学会の論文
- 2002-12-23
著者
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Inoue Yuichi
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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弥武 経也
昭和産業(株)技術部
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Inoue Y
Showa Sangyo Co. Ltd.
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Inoue Y
Solar Energy Research Group Institute For Physical And Chemical Research
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Inoue Yorinao
Photosynthesis Research Lab.
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INOUE Yasushi
Showa Sangyo Co., Ltd.
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YASUTAKE Nozomu
Showa Sangyo Co., Ltd.
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OSHIMA Yoshie
Showa Sangyo Co., Ltd.
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YAMAMOTO Yoshie
Showa Sangyo Co., Ltd.
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TOMITA Tetsuji
Showa Sangyo Co., Ltd.
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MIYOSHI Shinsuke
Showa Sangyo Co., Ltd.
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YATAKE Tsuneya
Showa Sangyo Co., Ltd.
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Yoshino Ichiro
The Department Of Surgery Ii Faculty Of Medicine University Of Occupational And Environmental Health
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YASUTAKE Nozomu
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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Tomita T
Showa Sangyo Co. Ltd.
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Yamamoto Yoshie
Showa Sangyo Co. Ltd.
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Yatake T
Showa Sangyo Co. Ltd.
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Yatake Tsuneya
Research And Development Center Showa Sangyo Co. Ltd.
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Yatake Tsuneya
R & D Center Kashima Division Showa Sangyo Co. Ltd.
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Oshima Yoshie
Showa Sangyo Co. Ltd.
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Yasutake Nozomu
Science Of Biological Resource The United Graduate School Of Agricultural Science Gifu University
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Miyoshi Shinsuke
Showa Sangyo Co. Ltd.
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