Purification and Some Properties of S-Hemolysin Produced by Streptomyces sp. Strain No. A-6288
スポンサーリンク
概要
- 論文の詳細を見る
A new cytolytic toxin, designated as S-Hemolysin, was found in the culture filtrate of Streptomyces sp. strain No. A-6288, isolated from a soil sample. The molecular weight of S-Hemolysin was estimated to be 10,000 by SDS-polyacrylamide gel electrophoresis and to be 20,000 by Sephadex G-100. S-Hemolysin is a glycoprotein that is composed of 102 amino acid residues with 11.6% glucose, and the isoelectric point is around pH 5.8. The phospholipase C activity of S-Hemolysin was specific for the following substrates in this order : sphingomyelin > lysophosphatidylethanolamine > lysophosphatidylcholine > phosphatidylethanolamine > phosphatidylcholine. S-Hemolysin had hemolytic activity against rabbit, human, and sheep erythrocytes, but did not cause aggregation of human platelets. These activities were accelerated with Mg^<2+>, Mn^<2+>, and Co^<2+> ions and inhibited by the addition of Ca<2+>, Cu^<2+>, and Zn^<2+> ions. This enzyme was shown to be different from the known bacterial phospholipase C.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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UYEDA Masaru
Laboratory of Pharmaceutical Microbiology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto
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Uyeda Masaru
Laboratory Of Pharmaceutical Microbiology Faculty Of Medical And Pharmaceutical Sciences Kumamoto Un
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Uyeda Masaru
Shokei University
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Uyeda Masaru
Laboratory Of Medicinal Microbiology Faculty Of Pharmaceutical Sciences Kumamoto University
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Suzuki K
Univ. Tokyo Tokyo
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SUZUKI KEITAROU
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences Kumamoto University
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Suzuki Keitarou
Laboratory Of Medicinal Microbiology Faculty Of Pharmaceutical Sciences Kumamoto University
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Udayama Manabu
Faculty Of Pharmaceutical Sciences Kumamoto University
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Uyeda Masaru
Department Of Pharmaceutical Microbiology Faculty Of Medical And Pharmaceutical Sciences Kumamoto Un
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SIDDIQUE Tariq
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University
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Matsunaga Kazuhito
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University
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Ehara Tsuyuko
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University
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Sakumura Yuko
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University
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Siddique Tariq
Laboratory Of Medicinal Microbiology Faculty Of Pharmaceutical Sciences Kumamoto University
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Sakumura Yuko
Laboratory Of Medicinal Microbiology Faculty Of Pharmaceutical Sciences Kumamoto University
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Ehara Tsuyuko
Department Of Physiology Saga Medical School
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Suzuki Kazuharu
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Matsunaga Kazuhito
Laboratory Of Medicinal Microbiology Faculty Of Pharmaceutical Sciences Kumamoto University
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