Characterization of Recombinant Yeast Exo-β-1,3-Glucanase (Exg 1p) Expressed in Escherichia coli Cells
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概要
- 論文の詳細を見る
Yeast exo-β-1,3-glucanase gene (EXG1) was expressed in Escherichia coli and the recombinant enzyme (Exg1p) was characterized. The recombinant Exg1p had an apparent molecular mass of 45 kDa by SDS-PAGE and the enzyme has a broad specificity for β-1,3-linkages as well as β-1,6-linkages, and also for other β-glucosidic linked substrates, such as cellobiose and pNPG. Kinetic analyses indicate that the enzyme prefers small substrates such as laminaribiose, gentiobiose, and pNPG rather than polysaccharide substrates, such as laminaran or pustulan. With a high concentration of laminaribiose, the enzyme catalyzed transglucosidation forming laminarioligosaccharides. The enzyme was strongly inhibited with high concentrations of laminaran.
- 社団法人日本農芸化学会の論文
- 2001-06-23
著者
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Abe Kohji
Laboratory Of Nutrition Chemistry
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Abe K
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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NAKAJIMA Tasuku
Division of Life Science, Graduate School of Agricultural Science, Tohoku University
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Maruyama Yutaka
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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Abe K
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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SUZUKI Kanako
Divison of Applied Life Science, Graduate School of Agricultural Science, Tohoku University
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YABE Tomio
Divison of Applied Life Science, Graduate School of Agricultural Science, Tohoku University
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ABE Keietsu
Divison of Applied Life Science, Graduate School of Agricultural Science, Tohoku University
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Nakajima Tasuku
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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Yabe Tomio
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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Suzuki Kanako
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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Nakajima Tasuku
Division Of Life Science Graduate School Of Agricultural Science Tohoku University
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Abe Keietsu
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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