Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1α (EF-1α) of Neurospora crassa

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概要

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• The tef-1 gene encoding translation elongation factor lα was cloned from the ascomycete fungus Neurospora crassa. The sequences of genomic DNA and cDNA clones showed that the tef-1 gene contained one ORF of 1380 bp length that is interrupted by three short introns. The deduced polypeptide contained 460 amino acid residues, and the sequence had a high similarity with those of EF-lα polypeptides from other species. The level of tef-1 mRNA was low in conidia but high in growing cells. When mycelia were transferred to poor nutrient media, the level of tef-1 gene mRNA decreased remarkably. The pattern of tef-1 expression was similar to the expression of genes for ribosomal proteins. The tef-1 gene was mapped between arg-3 and leu-4 loci on linkage group I by restriction fragment length polymorphism mapping. Southern blot analysis showed that Neurospora genomic DNA contained only one copy of the tef-1 gene in a genome.

著者

• INOUE Hirokazu

  Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University

• Inoue Hirokazu

  Laboratory Of Genetics Department Of Regulation Biology Faculty Of Science Saitama University

• ICHI-ISHI Akihiko

  Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University

関連論文

• Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa
• Functional Analysis of an Endo-1,6-β-D-glucanase Gene (neg-1) from Neurospora crassa
• Cloning, nucleotide sequence, and expression of tef-1, the gene encoding translation elongation factor 1α (EF-1α) of Neurospora crassa

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