Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa
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概要
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The un-17 mutant was originally isolated as an irreparable temperature-sensitive (ts) mutant in Neurospora crassa. Early experiments showed that cells of this mutant immediately stopped growing and died when the temperature of the culture was shifted from a permissive temperature (25°C) to non-permissive temperature (35°C). This ts phenotype is suppressed by addition of cycloheximide or in some conditions of growth repression. Even at the permissive temperature, it shows a female sterile phenotype and is deficient in production of exocellular superoxide dismutase SOD4 (EC 1.15.1.1). By searching for a DNA fragment that complements the ts phenotype of the un-17 mutant from a N. crassa genome library, we found the un-17 gene. The cloned un-17 gene encodes a homolog of the Saccharomyces cerevisiae poly(A) polymerase (PAP). The un-17 mutant had a one-base substitution mutation in the gene. The cloned un-17 genes from the wild-type strain and the un-17 mutant were introduced into both the un-17 mutant and wild-type strain. The un-17 mutant introduced by un-17 DNA from the wild-type strain showed recovery of both the ts and female sterile phenotypes. Moreover, the purified product derived from the wild-type strain showed PAP activity in vitro. These findings indicate that the un-17 mutant carries a ts mutation in the gene encoding PAP.
- 2007-12-25
著者
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INOUE Hirokazu
Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University
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Ishii Chizu
Laboratory Of Genetics Department Of Regulation-biology Faculty Of Science Saitama University
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Ishii Chizu
Laboratory Of Genetics Department Of Regulation Biology Faculty Of Science Saitama University
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Inoue Hirokazu
Laboratory Of Genetics Department Of Regulation-biology Faculty Of Science Saitama University
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Inoue Hirokazu
Laboratory Of Genetics Department Of Regulation Biology Faculty Of Science Saitama University
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TANAKA Shuuitsu
Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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TAKAYANAGI Noriko
Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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MURASAWA Kennichi
Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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Tanaka Shuuitsu
Laboratory Of Genetics Department Of Regulation-biology Faculty Of Science Saitama University
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Takayanagi Noriko
Laboratory Of Genetics Department Of Regulation-biology Faculty Of Science Saitama University
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Murasawa Kennichi
Laboratory Of Genetics Department Of Regulation-biology Faculty Of Science Saitama University
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