Purification and Some Properties of Two Wall-associated (β1,3)Glucanases from Neurospora crassa Cells(Biological Chemistry)

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概要

• 論文の詳細を見る

• Two cell wall associated (β1,3)glucanases in Neurospora crassa, 1,3-glucanase-I and II, were solubilized by autolysis of the cell walls with 2M sodium chloride, and purified by laminarioligosaccharide AH-sepharose affinity chromatography followed by preparative disc gel electrophoresis. The 1,3-glucanase-I has a strict specificity for (β1,3)glucan and produces D-glucose by an exo-type mode of action. The 1,3-glucanase-II has specificities for β-glucoside in addition to (β1,3)glucan. The 1,3-glucanase-I is more resistant to pH change and metal ions than the 1,3-glucanase-II.

• 社団法人日本農芸化学会の論文
• 1986-10-23

著者

• Matsuda Kazuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)iwaki-

• Matsuda Kazuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• NAKAJIMA TASUKU

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• KOBAYASHI Mikihiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• Kobayashi M

  Biophotonics Information Laboratories Yamagata Advanced Technology Research And Development Center

• Kobayashi Mikihiko

  Jissen Women's Univ.

• Kobayashi Mikihiko

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• HIURA Nozomi

  Department of Agricultural Chemistry, Tohoku University

• Nakajima T

  Hoechst Japan Ltd. Saitama Jpn

• Nakajima Tasuku

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Matsuda K

  Iwaki Meisei Univ. Fukushima Jpn

• KOBAYASHI Masaki

  Yamagata Advanced Technology Research and Development Center

• Hiura Nozomi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Matsuda Kazuo

  Department Of Agricultural Biochemistry Faculty Of Agriculture Tohoku University

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