An Isomaltotriose-producing Dextranase from Flavobacterium sp. M-73: Purification and Properties
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概要
- 論文の詳細を見る
An isomaltotriose-producing dextranase II, detected in the culture supernatant of Flavobacterium sp. M-73, was purified to an electrophoretically pure state. Successive chromatography on hydrophobic columns of Amberlite CG-50 and aminooctyl-Sepharose was very effective as the first step of purification. Further purification of the enzyme was performed by affinity column chromatography on isomaltotriose-Sepharose and preparative polyacrylamide gel electrophoresis. The purified enzyme was shown to be a monomer and had a molecular weight of 114, 000. Dextranase II was most active at pH 7.0 and 35°C. It was stable at 4°C for 24hr over a pH range of 6.5-12.0 and up to 35°C on heating for 10 min. This enzyme had a strict specificity for consecutive α-1, 6-glucosidic linkages and readily hydrolyzed clinical dextran and Sephadex gels. The degree of hydrolysis of clinical dextran was 31% expressed as apparent conversion into D-glucose. The amount of isomaltotriose in the hydrolyzate was determined to be 63%.
- 社団法人 日本農芸化学会の論文
著者
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Takagi Shiro
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Mitsuishi Yasushi
Department Of Molecular Biology National Institute Of Bioscience And Human Technology
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Kobayashi Mikihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda Kazuo
Department Of Agricultural Biochemistry Faculty Of Agriculture Tohoku University
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SHIOTA Masao
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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