Biosynthesis of Xyloglucan in Suspension-Cultured Soybean Cells. Synthesis of Xyloglucan from UDP-Glucose and UDP-Xylose in the Cell-Free System
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概要
- 論文の詳細を見る
When UDP-[^<14>C]glucose or UDP-[^<14>C]xylose was incubated with a particulate fraction from soybean cells, radioactive polymers were synthesized. On digestion with Aspergillus oryzae enzymes, these polymers gave ^<14>C-monosaccharides and a ^<14>C-disaccharide with chromatographic and electrophoretic mobilities indistinguishable from those of authentic isoprimeverose (6-O-α-D-xylopyranosyl-D-glucopyranose) . The disaccharide consisted of xylose and glucose, and the latter was located at the reducing end. Evidence that the disaccharide is isoprimeverose was provided by methylation analysis. Hydrolysis of the methylated disaccharide yielded 2,3,4-tri-O-methyl-D-xylose and 2,3,4-tri-O-methyl-D-glucose. Thus, incorporation of radioactivity into isoprimeverose, the smallest structural unit of xyloglucan, suggests that xyloglucan is synthesized in vitro from UDP-glucose and UDP-xylose.
- 日本植物生理学会の論文
著者
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Matsuda Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)iwaki-
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Hayashi Takahisa
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present)department Of
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Hayashi Takahisa
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda Kazuo
Department Of Agricultural Biochemistry Faculty Of Agriculture Tohoku University
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