Degradation of Xyloglucan by Wall-bound Enzymes from Soybean Tissue I. Occurrence of Xyloglucan-degrading Enzymes in Soybean Cell Wall

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An enzyme preparation that catalyzes the degradation of xyloglucan was obtained by extraction of the cell walls of soybean hypocotyls with a buffer containing 1.0 M NaCl. The enzyme preparation was shown to catalyze two-step degradation of xyloglucan. The polysaccharide was first degraded into comparatively large fragments, which were then further degraded into monosaccharides. In order to elucidate the mode of degradation of the xyloglucan during cell growth, the activities of xyloglucan-degrading enzymes of soybean-hypocotyl segments were assayed at different stages of elongation. The total activities of the degrading enzymes were lower in the elongating regions than in the non-elongating regions. However, high levels of endo-β-1,4-glucanase were found in the elongating regions. These results suggest that xyloglucan is hydrolyzed by endo-β-1,4-glucanase into comparatively large fragments at the initial stage of growth and the resulting fragments are further degraded into monosaccharides during cell elongation.
日本植物生理学会の論文