β-Galactosidase and Its Significance in Ripening of "Saijyo" Japanese Persimmon Fruit(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The fruit extracts of ripening cv. Japanese Persimmon, "Saijyo", contained a number of glycosidases and glycanases. Among them, β-galactosidase appeared to be the most significant, and the activity increased in parallel with tissue ripening. Persimmon β-galactosidase was presented in at least three isoforms, β-galactosidase-I (pI = 4.88), β-galactosidase-II (pI = 6.76), and β-galactosidase-III (pI = 7.05). Β-Galactosidase-III had exo-type galactanase activity, while the others did not. The activity of endo-type glycanases was a maximum in immature green or yellow fruits. The firmness of the pulp tissue decreased dramatically, and the amount of water-soluble polysaccharide (WSS) increased. The enzyme activities of exo-type glycosidases, especially β-galactosidase, appeared maximal in mature red fruits. The amount of extractable pectin remained unchanged, although the galactose content of the high-molecular-weight fraction in WSS decreased dramatically. These results suggest that the ripening of persimmon was caused by the solubilization of pectic polysaccharide by endo-type glycanases and digestion by exo-type glycosidases. Β-Galactosidase, in particular, seemed to play a major role in ripening the fruit.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
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Maeda H
Tsukuba R&d Center Fuji Oil Co Ltd.
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Maeda H
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Maeda Hirokazu
Protein Development Dept. Ii Hannan R&d Center Fuji Oil Co.ltd.
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Nagamatsu Yasunori
Faculty Of Applied Science Hiroshima University
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Nagamatsu Yasunori
Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University
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永松 康徳
広島大 生物生産
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NAKAMURA Akihiro
Tsukuba R&D Center, Fuji Oil Co., Ltd.
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MAEDA Hirokazu
Tsukuba R&D Center, Fuji Oil Co., Ltd.
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MIZUNO Misae
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University
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KOSHI Yoko
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University
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Koshi Yoko
Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University
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Mizuno Misae
Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University
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Nagamatsu Y
Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University
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Nakamura A
Tsukuba R&d Center Fuji Oil Co Ltd.
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Nakamura Akihiro
Tsukuba R & D Center, Fuji Oil Co., Ltd.
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NAKAMURA Akihiro
Department of Physics, College of Liberal Arts
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NAKAMURA Akihiro
Department of Physics, College of Liberal Arts Kagoshima University
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Maeda Hirokazu
Tsukuba R & D Center, Fuji Oil Co., Ltd.
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