Cloning, Sequencing, and Expression of the Bombyx mori Receptor for Bacillus thuringiensis Insecticidal CryIA (a) Toxin

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概要

• 論文の詳細を見る

• Bacillus thuringiensis strains produce insect-specific Bt toxins. Bt CryIA(a) toxin binds to a 175-kDa glycoprotein (BtR175) on the microvillus membranes of columnar cells in the Bombyx mori midgut and causes lysis of the cells. BtR175 was purified, and its cDNA was cloned. The cDNA encodes a newly identified 193.3-kDa preproprotein form of BtR175 that includes nine extracellular cadherin repeats, a 23.5-kDa membrane-proximal domani, a membranespanning region, and a 13.6-kDa cytoplasmic domain. Spodoptera frugiperda cells transfected with a recombinant baculovirus DNA carrying the cDNA produced a 175-kDa protein that reacted with anti-BtR antibodies and the Bt CryIA(a) toxin.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• MIYOSHI YOKO

  Department of Pediatrics, Osaka University Graduate School of Medicine

• Nagamatsu Yasunori

  Faculty Of Applied Science Hiroshima University

• Nagamatsu Yasunori

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• 永松 康徳

  広島大 生物生産

• TODA Satoshi

  Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University

• KOGURE Masato

  Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University

• Toda S

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• Miyoshi Y

  Hiroshima Univ. Higashi‐hiroshima

• Kogure Masato

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• Nagamatsu Y

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• KOIKE Takashi

  Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University

• SHIGEMATSU Sara

  Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University

• Miyoshi Yoko

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• Koike Takashi

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

• Shigematsu Sara

  Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University

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