Purification and Characterization of Novel Whey Glycoprotein WGP-88 Which Binds to a Monoclonal Antibody to PAS-4 Glycoprotein in the Bovine Milk Fat Globule Membrane
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概要
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A monoclonal antibody to the PAS-4 glycoprotein (78 kDa) of the bovine milk fat globule membrane (MFGM) specifically recognized PAS-4, and was named KAS4. A component recognized by KAS4 was found in whey protein, this being a glycoprotein of 88 kDa by SDS-PAGE and named WGP-88. WGP-88 was purified and characterized in comparison with PAS-4. WGP-88 had apparent pI values of 6.45 and 6.39, while those of PAS-4 were 7.39 and 7.35. Neuraminidase digestion shifted the pI values of WGP-88 to 6.57 and of PAS-4 to 7.52. WGP-88 was rich in polar amino residues (44.9 mol%), while PAS-4 was abundant in nonpolar amino acid residues (48.7 mol%). WGP-88 contained 17.1% of carbohydrate and PAS-4 had 7.20%. The results of reductive hydrolysis, Nglycanase digestion, and a lectin blot analysis suggested that N- and O-linked sugar chains were contained in both glycoproteins. WGP-88 and PAS-4 had a different N-terminal amino acid sequence. WGP-88 and PAS-4 respectively inhibited competitively the binding of KAS4 to PAS-4 and WGP-88. Our studies revealed WGP-88 recognized by KAS4 mAb to be a novel whey protein and to have different biochemical properties from those of PAS-4.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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Azuma Norihiro
Department of Bioproductive Science, Faculty of Agriculture, Utsunomiya University
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Hwangbo Sik
Department Of Applied Biochemistry College Of Agriculture Utsunomiya University
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Azuma N
Department Of Biological Resource Chemistry Kyoto Prefectural University
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Azuma Norihiro
Department Of Agricultural Chemistry The University Of Tokyo
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KANNO Choemon
Department of Animal Science, Utsunomiya University
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KURISAKI Jun-ichi
Department of Agricultural Chemistry, The University of Tokyo
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Kanno C
Department Of Applied Biochemistry Utsunomiya University
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Kanno Choemon
Department Of Animal Science Utsunomiya University
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Kurisaki Jun-ichi
Department Of Animal Products National Institute Of Animal Industry
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KURISAKI Jun-ichi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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