Properties of Glycoprotein Fractions of Bovine Milk Fat Globule Membrane Selectively Solubilized with Lithium Diiodosalicylate and Partitioned with Phenol
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Most of the glycoproteins were extracted selectively from bovine milk fat globule membrane (MFGM) and MFGM-apoprotein with lithium diiodosalicylate (LDS). By partitioning the LDSextracts with phenol, the glycoproteins were separated into two phases: the upper LDS-aqueous phase and the lower phenol phase. Special attention was paid to the glycoproteins partitioned in the latter and the characteristics of the fractions were compared. Of the major glycoproteins stained with periodic acid-Schiff reagent (PAS), PAS-1, 2, and 3 bands were extracted in the LDS-aqueous phase and PAS-4, 6 and 7 bands into the phenol phase. PAS-5 was not extracted with LDS. Of glycopeptides constituting the soluble glycoprotein, PAS-8 and 9 bands were partitioned into the aqueous phase, but PAS-10 and 11 into the phenol phase. The glycoprotein partitioned in the phenol fraction was relatively rich in hexoses and had fewer hydrophilic amino acid residues, and a high absorption coefficient (13.6), while that of the LDS-aqueous phase was rich in sialic acid and hydrophilic amino acid residues and had a lower absorption coefficient (4.1).
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