Hormonal Effect on the Formation of Lipid-Saccharide Intermediates for N-Linked Glycoprotein Biosynthesis of Lactating Bovine Mammary Gland in Organ Culture: Evidence of Inhibitory Action of Hydrocortisone(Biological Chemistry)

概要

論文の詳細を見る
The effects of prolactin (P), hydrocortisone (F), and insulin (I) on the formation of lipid-saccharide intermediates concerned in the N-linked glycoprotein biosynthesis of lactating bovine mammary explants in organ culture were examined using radioactive mannose (Man). The response of was fast (<20 hr), while that of P and I was delayed (>72 hr). F at concentrations varied from 10^<-3> to 10 μg/ml decreased the incorporation of radioactivity from [^<14>C]Man into the lipid-linked saccharides (C/M), lipid-linked oligosaccharides (C/M/W), and protein fraction by about 25〜30%. On the other hand, at the range of 10^<-4> to 5 μg/ml, I had a stimulatory effect and P did not affect the N-glycosylation of mammary explants. The stimulatory effect of I, however, was negated by with F. Partial inhibition of F was shown by even 10 ng/ml. The inclusion of F into the pre-incubation medium significantly inhibited the [^<14>C]Man incorporation in the C/M/W and protein fractions rather than in the C/M fraction. The results suggest that F inhibits the formation of the lipid-linked saccharide intermediates for N-glycosylated glycoprotein in lactating bovine mammary explants.
社団法人日本農芸化学会の論文
1988-10-23