Purification of Membrane-bound Lactoferrin from the Human Milk Fat Globule Membrane

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概要

• 論文の詳細を見る

• Although lactoferrin is known as a basic soluble glycoprotein, the presence of the membrane-bound form of this protein has also been demonstrated in human milk. Membrane-bound lactoferrin was extracted from the human milk fat globule membrane with a detergent mixture of 1% Tween-20,0.5% C_<12>E_8,and 0.5 M KCl in 20 mM Tris-HCl (pH 7.4). Lactoferrin in the detergent-soluble fraction was purified by affinity chromatography with Concanavalin A and by hydrophobic chromatography with phenyl-Superose. The purified protein gave a single band of 80 kDa by SDS-PAGE. Its N-terminal amino acid sequence was consistent with that of human lactoferrin.

• 社団法人日本農芸化学会の論文
• 2000-03-23

著者

• Yu Jae-hyun

  韓国

• LEE Chi-Ho

  Department of Food Science and Biotechnology of Animal Resources, Konkuk University

• Lee Chi-ho

  Department Of Food Science And Biotechnology Of Animal Resources Konkuk University

• Azuma Norihiro

  Department Of Agricultural Chemistry The University Of Tokyo

• Azuma Norihiro

  Department Of Applied Biochemistry Utsunomiya University

• Kanno Choemon

  Department Of Animal Science Utsunomiya University

• Lee Chiho

  Department Of Food Science And Biotechnology Of Animal Resources Konkuk University

• Yu Jaehyeun

  Department Of Dairy Science Kon-kuk University

• CHO JinKook

  Department of Applied Biochemistry, Utsunomiya University

• LEE ChiHo

  Faculty of Animal Life Science, Kon-kuk University

• YU JaeHyeun

  Faculty of Animal Life Science, Kon-kuk University

• Cho Jinkook

  Department Of Applied Biochemistry Utsunomiya University:animal Resources Research Center

• Cho Jin-Kook

  Department of Applied Biochemistry, Utsunomiya University

• Lee Chi-Ho

  Faculty of Animal Life Science, Kon-kuk University

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