肺動脈平滑筋のホルボールエステルによるCa非依存性収縮にはCdc42が関与する(薬理学)
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概要
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ホルボールエステルにより発生するCa^<2+>非依存性の収縮に, 低分子GTP結合タンパク質Rho GTPaseファミリーがどのように関与するかを, ブタ肺動脈平滑筋を用いて検討した.Ca^<2+>除去溶液(1mM EGTA含む)中にCキナーゼ(PKC)活性化薬である12-deoxyphorbol 13-isobutyrate (DPB, 1μM)を投与すると持続性の収縮が発生したが, この収縮は電位依存性Ca^<2+>チャネル抑制剤verapamilおよびRhoキナーゼ阻害剤Y27632では抑制されなかった.ウェスタンブロッティングにより, ブタ肺動脈平滑筋にはPKCα, PKCε, PKCζ, そしてRhoAとCdc42の発現が観察された.また, DPB処置によりPKCαとPKCεは細胞質から細胞膜へ移行し, さらにCdc42を細胞質から細胞膜へ移行させた.同様の結果がCdc42のpull-downアッセイ実験でも確認された.しかし, RhoAについてはDPB処置によるトランスロケーションは見られなかった.Norepinephrine (10μM)はRhoAを細胞質から細胞膜へ有意に移動させたが, Cdc42やPKCに対するトランスロケーション作用は示さなかった.以上の結果からホルボールエステルは低分子GTP結合蛋白Rhoファミリー, 特にCdc42を介してCa^<2+>非依存性の収縮を起こす可能性が示唆された.
- 社団法人日本獣医学会の論文
- 2005-08-25
著者
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Kim Yoon
Department of Obstetrics and Gynecology, Microbiology and Immunology, Chonnam National University Me
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LEE Chang
Department of Oral Biology, Yonsei University College of Dentistry
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Lee Chang
Department Of Physiology & Immunology School Of Medicine Konkuk University
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Choi W.-h.
韓国
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CHOI Yoon
Department of Astronomy, Graduate School of Science, The University of Tokyo
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Choi Won
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Woo Nam
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Kim Bokyung
Department Of Physiology & Immunology School Of Medicine Konkuk University
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Kim B.
韓国
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Kim Bokyung
College Of Medicine Konkuk University
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Kim Jin-hyun
Department Of Veterinary Pathology College Of Veterinary Medicine And Agricultural Biotechnology Seo
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KIM Junghwan
Department of Physical Therapy, College of Natural Science, Yongin University
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LEE Youn
Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konku
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KIM Jaeheung
Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konku
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CHO SungIl
Department of Physiology and Anesthesiology, College of Medicine, Institute of Biomedical Science an
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Lee Chang
Department Of Animal Science Utsunomiya University
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Choi Won
Department Of Agricultural Chemistry Kyushu University
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Lee Youn
Department Of Physiology College Of Medicine Institute Of Biomedical Science And Technology Konkuk U
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Choi Yoon
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Cho Sungil
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Kim Bokyung
韓国
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Kim Junghwan
Department Of Physiology & Immunology School Of Medicine Konkuk University
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Kim Junghwan
Department Of Physical Therapy College Of Natural Science Yongin University
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Kim Yoon
Department Of Anesthesiology And Pain Medicine Chungnam National University Hospital
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Kim Bongtae
Department Of Veterinary Pathology College Of Veterinary Medicine And School Of Agicultural Biotechn
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Kim J.
Department Of Physiology & Immunology School Of Medicine Konkuk University
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Cho S.
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Lee Youn
Department Of Hospital Pathology St. Mary's Hospital The Catholic University Of Korea College O
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Kim Junghwan
Dep. Of Physical Therapy Coll. Of Public Health & Welfare Yongin Univ.
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Kim Yoon
Department Of Physiology And Anesthesiology College Of Medicine Institute Of Biomedical Science And
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Lee Chang
Departlnent Of Biology College Of Natural Sciences Kyungpook National University Korea
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Choi Won-Ho
Department of Physiology and Anesthesiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University
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Choi Won-Ho
Department of Electronics Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea
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CHOI Yoon
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan
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KIM Bokyung
Department of Medicine, Konkuk University
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Choi Won-Ho
Department of Computer Engineering, University of Kyungnam
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