Cloning and Sequencing of the Deacetylase Gene from Vibrio alginolyticus H-8
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概要
- 論文の詳細を見る
A gene encoding deacetylase DA1 that is specific for N, N′-diacetylchitobiose was cloned using the shot-gun method with pUC118 and sequenced. The open reading frame encoded a protein of 427 amino acids including the signal peptide. The molecular mass of the mature enzyme estimated from the amino acid sequence data was 44.7 kDa, which is approximately similar to that, estimated by SDS-PAGE(48.0 kDa), of the purfied enzyme reported previously. The N-terminal amino acid sequence deduced from the cloned deacetylase gene showed partial sequence homology with the NodB protein from Rhizobium sp.(37% identity) and chitin deacetylase from Mucor rouxii(28%). It contained a domain, which showed homology with a chitin-binding domain of chitinase A from Bacillus circulans(39%).
- 社団法人日本生物工学会の論文
- 2000-11-25
著者
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Etoh Hideo
Faculty of Agriculture, Shizuoka University
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Ono T
Shigei Medical Research Inst. Okayama
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Ohta T
Numazu Industrial Res. Inst. Shizuoka Prefecture Numazu‐shi Jpn
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OHTA TOSHIYA
Numazu Industrial Research Institute of Shizuoka Prefecture
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Etoh Hideo
Faculty Of Agriculture Shizuoka University
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OHISHI KAZUO
United Graduate School of Agricultural Sciences, Gifu University(Shizuoka University)
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MURASE KOHJI
Faculty of Agriculture, Shizuoka University
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Murase Kohji
Faculty Of Agriculture Shizuoka University
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Ohishi K
United Graduate School Of Agricultural Sciences Gifu University
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Ohishi Kazuo
United Graduate School of Agricultural Sciences, Gifu University
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