Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells
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概要
- 論文の詳細を見る
The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula × P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.
- 社団法人 日本農芸化学会の論文
著者
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Taniguchi Toru
Forestry and Forest Products Research Institute, Forest Bio-research Center
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Sakka Kazuo
Graduate School Of Bioresources Mie Univ.
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OGAWA Naoto
Faculty of Agriculture, Kyushu University
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Kimura Tetsuya
Graduate School Of Human & Environmental Studies Kyoto University
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Ohta T
Numazu Industrial Res. Inst. Shizuoka Prefecture Numazu‐shi Jpn
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MIYASHITA Kiyotaka
National Institute for Agro-Environmental Sciences
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OHMIYA Yasunori
Forest Tree Breeding Center, FFPRI
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OHMIYA Kunio
Graduate School of Bioresources, Mie University
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ONO Toshiro
Graduate School of Bioresources, Mie University
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ITAHANA Naoei
Forest Tree Breeding Center
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