Efficient Embryogenic Callus Induction and Plant Regeneration from Embryonic Axis Explants in Quercus acutissima
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概要
- 論文の詳細を見る
Embryogenic callus of Quercus acutissima was successfully induced from embryogenic cultures, and plants were regenerated from the callus. The development of the techniques involved will allow mass propagation and gene transformation in this species. Embryogenic cultures were formed from embryonic axis explants (i. e., embryos without cotyledons) excised from immature embryos, after culture on Murashige and Skoog (MS) medium containing indolebutyric acid and benzyladenine. Attempts to induce embryogenic cultures from cotyledon explants were unsuccessful. Embryogenic calli were induced at high frequency from embryogenic cultures on MS medium containing 2,4-dichlorophenoxyacetic acid. However, benzyladenine inhibited embryogenic callus formation. Somatic embryo development from embryogenic calli occurred on MS medium in all of the seven cell lines tested. Germination of somatic embryos was induced on half strength MS medium without plant growth regulators. Finally, acclimated plants growing in soil were obtained.
- 一般社団法人日本森林学会の論文
- 2001-05-16
著者
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Taniguchi Toru
Forestry and Forest Products Research Institute, Forest Bio-research Center
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Kondo Teiji
Forestry and Forest Products Research Institute, Forest Bio-research Center
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Kondo Teiji
Forest Bio-research Center Forestry And Forest Products Research Institute (ffpri)
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Taniguchi Toru
Forest Bio-research Center Forestry And Forest Products Research Institute (ffpri)
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Okamura Masanori
Forest Tree Breeding Center
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Okamura Masanori
Forest Tree Breeding Center:(present Address)kyushu Regional Breeding Office Forest Tree Breeding Ce
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