A MODIFIED METHOD FOR DETERMINATION OF CELLULASE ACTIVITY IN FOREST SOIL
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概要
- 論文の詳細を見る
The optimum conditions for the determination of the cellulase activity of forest soil were defined as follows: One g of wet soil is placed into a test tube (20×150 mm), and 0.2 ml of toluene is added. After the mixture has been allowed to stand for 10 min, 10 ml of 0.2 M sodium acetate buffer solution containing 10 mg of CMC per ml is added. The mixture is stirred by a Thermo-Mixer, covered with a @Parafilm,@ and is incubated at 50℃ for 4 h in a shaking incubator. The reaction solution is separated by centrifugation, and 0.5 g of Polychlal AT is added, to remove colored humus. Centrifugation is repeated, to remove Polychlal AT. The glucose in the decolorized reaction solution is determined according to the glucostat method.
- 社団法人日本土壌肥料学会の論文
著者
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Kanazawa Shinjiro
Faculty of Agriculture, Kyushu University
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MIYASHITA Kiyotaka
National Institute for Agro-Environmental Sciences
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Kanazawa Shinjiro
Faculty Of Agriculture Department Of Agricultural Chemistry The University Of Tokyo
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Miyashita Kiyotaka
National Inst. For Agro-environmental Sciences
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Miyashita Kiyotaka
National Institute Of Agro-environmental Sciences
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