Cloning of Myrosinase cDNA from Wasabia japonica Matsum.

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概要

• 論文の詳細を見る

• Myrosinase (β-thioglucoside glucohydrolase, EC 3.2.3.1) is involved in pungency generation in wasabi (Wasabia japonica Matsum.). We isolated full-length cDNA (WjMY1) encoding myrosinase from the petiole of wasabi. The deduced polypeptide has 545 amino acids with a molecular weight of 62107. WjMY1 mRNA is accumulated in whole organs of the wasabi plant ; the order of expression is petiole≥rhizome>leaf. The order was found to be similar to that of the specific activity of myrosinase. This is the first report de- scribing the isolation of a myrosinase gene from wasabi.

• 社団法人　日本食品科学工学会の論文
• 2005-12-01

著者

• 久保井 徹

  静岡大 農

• HARA Masakazu

  Faculty of Agriculture, Shizuoka University

• KUBOI Toru

  Faculty of Agriculture, Shizuoka University

• Etoh Hideo

  Faculty of Agriculture, Shizuoka University

• Etoh H

  Faculty Of Agriculture Shizuoka University

• Etoh Hideo

  Faculty Of Agriculture Shizuoka University

• Etoh Hideo

  Faculty Of Pharmaceutical Sciences Meijo University

• Kuboi Toru

  Fac. Of Agriculture Shizuoka Univ.

• Kuboi Toru

  Faculty Of Agriculture Shizuoka University

• Hara Masakazu

  Faculty Of Agriculture Shizuoka University

• SAITOH Sanshiroh

  J-Oil Mills, INC.

• Saitoh Sanshiroh

  J-oil Mills Inc.

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