Biodegradation of Long-Chain n-Paraffins from Waste Oil of Car Engine by Acinetobacter sp.
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概要
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Microorganisms that degrade long-chain n-paraffins from used car engine oil were isolated from soil. For the screening, a fraction of n-paraffin prepared from car engine oil was applied as the sole carbon source. The strain was identified as Acinetobacter sp. The ability of the strain to assimilate long-chain n-paraffins was assessed and characterized. The strain mineralized long-chain n-paraffins (0.1% w/v) in the minimal medium after cultivation for 96 h and also reduced the weight of the waste oil added (1% w/v) by 20% after 72 h without an extracellular biosurfactant. When n-hexadecane was fed as substrate, 1-hexadecanol and 1-hexadecanoic acid were detected as the intermediates by gas chromatography/mass spectrometry. This indicates that the long-chain n-paraffins were metabolized via the terminal oxidation pathway of n-alkane.
- 社団法人日本生物工学会の論文
- 2001-01-25
著者
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Chung Seon-yong
Department Of Environmental Engineering College Of Engineering Chonnam National University
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Chung Seon-yong
Laboratory Of Microbiology Research Development Corporation:(present Address)college Of Engineering
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Kubo Motoki
Department Of Bioscience And Technology Faculty Of Science And Engineering Ritsumeikan University
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Kubo Motoki
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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YAMAMOTO Etsuo
Department of Polymer Science and Engineering, Kyoto institute of Technology
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Yamamoto Etsuo
Department Of Biology Tokyo Metropolotan University
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Yamamoto Etsuo
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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OHTA TOSHIYA
Numazu Industrial Research Institute of Shizuoka Prefecture
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KOMA Daisuke
Department of Bioscience & Technology, Faculty of Science & Engineering, Ritsumeikan University
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HASUMI Fumihiko
Department of Biochemistry, Numazu College of Technology
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KOME DAISUKE
Department of Bioscience & Technology, Faculty of Science & Engineering, Ritsumeikan University
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Koma Daisuke
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Hasumi F
Numazu Coll. Of Technol. Shizuoka Jpn
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