Improved Method for Pro-Urokinase Refolding with Inclusion Body from Recombinant Escherichia coli
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概要
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We developed an efficient inclusion body pro-urokinase refolding method from recombinant Escherichia coli. The protein was efficiently refolded when a heat treatment was applied to a protein denaturing solution containing guanidine hydrochloride. The total enzyme activity and the specific activity in response to the 50℃ heat treatment compared to normal method (25℃) were enhanced about 10 and 25%, respectively. Moreover, enhanced protein refolding was also observed in the case of a reduced protein concentration in the protein refolding solution. The result indicates that correct protein folding is closely related to the protein concentration in the refolding solution.
- 社団法人日本生物工学会の論文
- 1995-12-25
著者
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Kubo Motoki
Department Of Bioscience And Technology Faculty Of Science And Engineering Ritsumeikan University
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Nishi Akihiro
Scientific Instrument Development Department Tosoh Corporation
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Kubo Motoki
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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