Restraint Stress Induces Connexin-43 Translocation via α-Adrenoceptors in Rat Heart
スポンサーリンク
概要
- 論文の詳細を見る
Background: Immobilization (IMO) confers emotional stress in animals and humans. It was recently reported that IMO in rats induced translocation of connexin-43 (Cx43) to gap junctions (GJs) and attenuated arrhythmogenesis with GJ inhibition, and Cx43 translocation in the ischemic heart was also shown. Few reports show the contribution of adrenoceptors to Cx43 upregulation in cardiomyocytes, but the involvement of adrenoceptors and ischemia in Cx43 translocation in IMO remains elusive. Methods and Results: Male Sprague-Dawley rats underwent IMO and the ventricular distribution of Cx43 was examined by western blotting. IMO induced translocation of Cx43 to the GJ-enriched membrane fraction, with a peak at 60min. The IMO-induced Cx43 translocation was inhibited by pretreatment with the α1-adrenoceptor blockers, prazosin (1mg/kg, PO) and bunazosin (4mg/kg, PO), but not with either the β1-blocker, metoprolol (10mg/kg, IP), or the β1+2-blocker, propranolol (1mg/kg, PO). The translocation was inhibited by the nitric oxide, donor isosorbide dinitrate (100μg·kg-1·min-1, IV), possibly through sympathetic inhibition. Hypoxia inducible factor-1α was not redistributed by IMO. The β-blockers, but not the α-blockers, inhibited the premature ventricular contractions (PVCs) induced by IMO. Conclusions: Translocation of Cx43 to the GJ-enriched fraction occurs via the α1-adrenoceptor pathway, independently of ischemia. The β-adrenoceptor pathway contributes to the inducing of PVCs in IMO. (Circ J 2010; 74: 2693-2701)
- 社団法人 日本循環器学会の論文
- 2010-11-25
著者
-
Yoshida Ken-ichi
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
-
UNUMA Kana
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo
-
SHINTANI ISHIDA
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo
-
TSUSHIMA Kensuke
Department of Cardiovascular Medicine, University of Tokyo Hospital
-
SHIMOSAWA Tatsuo
Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo
-
UEYAMA Takashi
Departments of Anatomy and Cell Biology, Wakayama Medical University
-
Shintani Ishida
Department Of Forensic Medicine Graduate School Of Medicine University Of Tokyo
-
Unuma Kana
Department Of Forensic Medicine Graduate School Of Medicine University Of Tokyo
-
Unuma Kana
Department Of Forensic Medicine Graduate School Of Medicine The University Of Tokyo
-
Ueyama Takashi
Department Of Anatomy And Cell Biology Wakayama Medical University
-
Ueyama Takashi
Departments Of Anatomy And Cell Biology Wakayama Medical University
-
YAHAGI NAOKI
Department of Cardiovascular Surgery, National Cardiovascular Center
-
Tsushima Kensuke
Department Of Cardiovascular Medicine University Of Tokyo
-
Tsushima Kensuke
Department Of Cardiovascular Medicine University Of Tokyo Hospital
-
Shimosawa Tatsuo
Department Of Clinical Laboratory Faculty Of Medicine University Of Tokyo
-
Yoshida Ken-ichi
Department Of Forensic Medicine Graduate School Of Medicine University Of Tokyo
-
Yahagi Naoki
Department Of Cardiovascular Surgery National Cardiovascular Center
-
Yoshida Ken-ichi
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
-
SHINTANI-ISHIDA Kaori
Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo
-
Yahagi Naoki
Department Of Emergency And Critical Care Medicine University Of Tokyo Hospital
-
Shimosawa Tatsuo
Department Of Aging Control Medicine Juntendo University Graduate School Of Medicine
-
YOSHIDA Ken-ichi
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo
関連論文
- Ischemia Enhances Translocation of Connexin43 and Gap Junction Intercellular Communication, Thereby Propagating Contraction Band Necrosis After Reperfusion
- Polaprezinc (Zinc L-Carnosine) Is a Potent Inducer of Anti-oxidative Stress Enzyme, Heme Oxygenase (HO)-1 — a New Mechanism of Gastric Mucosal Protection
- D-Pinitol and myo-Inositol Stimulate Translocation of Glucose Transporter 4 in Skeletal Muscle of C57BL/6 Mice
- Aryl hydrocarbon receptor-mediated induction of the cytosolic phospholipase A_2α gene by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mouse hepatoma Hepa-1c1c7 cells(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Connexin-43 Redistribution and Gap Junction Activation During Forced Restraint Protects Against Sudden Arrhythmic Death in Rats
- The Significant Association of Genetic Polymorphisms in the KLF5 Gene with Coronary Artery Disease(Atherosclerosis, Clinical 9 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-447 The genetic polymorphisms in the insulin-resistance related genes as coronary risks(Preventive Medicine/Epidemiology/Education 4 (H) : PJ75)(Poster Session (Japanese))
- Molecular Cloning and Sequence Analysis of Two Distinct Halotolerant Extracellular Proteases from Bacillus subtilis FP-133
- Chronic Estrogen Supplementation Following Ovariectomy Improves the Emotional Stress-Induced Cardiovascular Responses by Indirect Action on the Nervous System and by Direct Action on the Heart
- OE-125 Estrogen Attenuates the Emotional Stress-induced Activation of the Sympathoadrenal-projecting Central Neurons in an Animal Model of Takotsubo Cardiomyopathy(Autonomic nervous system-1 (H) OE21,Oral Presentation (English),The 70th Anniversary Annual
- CHANGES IN CARDIAC α_1-ADRENOCEPTOR IN SPONTANEOUSLY HYPERTENSIVE RATS : Hypertension : 53 Annual Scientific Meeting, Japanese Circulation Society
- Effectiveness of Add-On Low-Dose Diuretics in Combination Therapy for Hypertension : Losartan/Hydrochlorothiazide vs. Candesartan/Amlodipine
- Regression of Gastroduodenal Mucosa-Associated Lymphoid Tissue Lymphoma after Eradication of Helicobacter pylori : A Case Report
- Low Grade MALToma and Early Cancer of the Stomach in a Patient with Helicobacter pylori Infection : A Case Report
- Two Cases of Renovascular Hypertension and Ischemic Renal Dysfunction : Reliable Choice of Examinations and Treatments
- Degradation and dephosphorylation of connexin43 in early myocardial infarction
- Suppression of Catechol-O-Methyltransferase Activity through Blunting of α_2-Adrenoceptor Can Explain Hypertension in Dahl Salt-Sensitive Rats
- Systemic Delivery of Retinoic Acid Receptor α Specific Agonist Inhibits In-Stent Restenosis by Inducing Differentiation of Smooth Muscle Cells(Vascular Smooth Muscle 1 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- High-throughput evaluation of aryl hydrocarbon receptor-binding sites selected via chromatin immunoprecipitation-based screening in Hepa-1c1c7 cells stimulated with 2,3,7,8-tetrachlorodibenzo-p-dioxin
- Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene of Sinorhizobium fredii USDA191 Required for myo-Inositol Utilization
- Functional myo-Inositol Catabolic Genes of Bacillus subtilis Natto Are Involved in Depletion of Pinitol in Natto (Fermented Soybean)
- No Involvement of the Nerve Growth Factor Gene Locus in Hypertension in Spontaneously Hypertensive Rats
- Implication of Protein Kinase C-α, δ, and εIsoforms in Ischemic Preconditioning in Perfused Rat Hearts
- Kruppel-like Transcription Factor KLF5 is a Key Regulator Governing Adipocyte Differentiation(Metabolic Syndrome (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Serum Response Factor (SRF) Cofactors in Smooth Muscle Differentiation
- Polaprezinc (Zinc L-Carnosine) Is a Potent Inducer of Anti-oxidative Stress Enzyme, Heme Oxygenase (HO)-1 : a New Mechanism of Gastric Mucosal Protection
- Persistent median artery in the hand : A report with a brief review of the literature
- MS2-2 Androgen metabolizing enzymes in the nervous system
- Binding of an Engineered 130-kDa Insecticidal Protein of Bacillus thuringiensis var.israelensis to Insect Cell Lines
- Importance of the Central Region of 130-kDa Insecticidal Proteins of Bacillus thuringiensis var. israelensis for Their Activity in Vivo and in Vitro
- Effects of Bacillus thuringiensis var.israelensis 20-kDa Protein on Production of the Bti 130-kDa Crystal Protein in Escherichia coli
- The Significant Association of the Genetic Polymorphisms of Werner Helicase and β2 Adrenergic Receptor with Susceptibility to Coronary Heart Disease
- Neurotrophin-3 gene expression negatively responds to serum through AP-1 transcription factor in rat cultured vascular smooth muscle cells
- OJ-512 A Zinc Finger Transcription factor δ EF1 Controls Smooth Muscle Cell Differentiation during Development and in Vascular Diseases(Vascular Smooth Muscle 2 (H) : OJ63)(Oral Presentation (Japanese))
- OE-161 Oral Retinoic Acid Receptor Alpha Agonist Inhibits In-Stent Restenosis(Restenosis after Angioplasty, Basic/Clinical 1 (IHD) : OE20)(Oral Presentation (English))
- Angiotensin II and Transforming Growth Factor Beta Induced Angiogenesis Associated Mts1 in Smooth Muscle Cells
- PPAR γ Ligand Dependent Interaction with Transcriptional Coactivators Control the Vascular Smooth Muscle Cell Differentiation
- An E-Box Binding Transcription Factor Controls Smooth Muscle Cell Differentiation Marker Genes
- Kruppel Like Zinc-Finger Trascription Factor 5 (KLF5) Madiate Immune Responses in Cardiovascular Remodeling
- Kruppel-Like Factor 5 (KLF5) and Peroxisome Proliferatesγ (PPARγ) Cooperatively Control Cellular Responses in Vascular Diseases
- Interaction between adrenomedullin and angiotensin II in DNA synthesis and extracellular matrix accumulation in cultured rat kidney interstitial cells
- Acetic acid improves the sensitivity of theophylline analysis by gas chromatography-mass spectrometry
- 0852 Blood Pressure Response to Mental Arithmetic in Patients with White-coat Hypertension
- 3 Targeting Oxidative Stress in Preventing Cardiovascular Complications in Metabolic Syndrome(New Horizons in the Metabolic Syndrome:-Molecular Mechanisms and Therapies,Plenary Session 4 (PL4) (H),The 70th Anniversary Annual Scientific Meeting of the Japa
- Protective Effect of Adrenomedullin in Renal Interstitial Fibrosis and Relation to Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1
- Organ-Protective Effects of Adrenomedullin
- Regional Hemodynamic Effects of Adrenomedullin in Wistar Rats: a Comparison with Calcitonin Gene-Related Peptide
- Caveolae are preferred sites for store-operated Ca^ linked to NO production
- Upregulation of Heme Oxygenase-1 in an Animal Model of Takotsubo Cardiomyopathy
- OE-139 Up-regulation of Cyclooxygenase 2 (COX-2) in an Animal Model of Takotsubo Cardiomyopathy(OE24,Autonomic Nervous System (H),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Estrogen Attenuated the Emotional Stress Responses in the Animal Model of Takotsubo Cardiomyopathy
- Emotional Stress Induces Transient Left Ventricular Hypocontraction in the Rat Via Activation of Cardiac Adrenoceptors : A Possible Animal Model of 'Tako-Tsubo' Cardiomyopathy
- P463 EFFECT OF WALKING ON AUTONOMIC ACTIVITY IN MILD HYPERTENSIVE PATIENTS
- A Case of Hypertrophic Obstructive Cardiomyopathy Associated With Autonomic Nervous Dysfunction
- Production of Nerve Growth Factor by Cultured Vascular Smooth Muscle Cells from Spontaneously Hypertensive and Wistar-Kyoto Rats.
- -0558-NERVE GROWTH FACTOR INCREASES IN SPONTANEOUSLY HYPERTENSIVE RATS
- On excellent elements of anti-integral extensions
- Downregulation of Calpastatin in Rat Heart after Brief Ischemia and Reperfusion
- Proteolysis of Erythrocyte-Type and Brain-Trype Ankyrins in Rat Heart after Postischemic Reperfusion
- Death in Head-down Position:An Autopsy Report with Reference to Physiological Mechanism
- A Characterization of Anti-Integral Extensions
- On integral domains whose divisorial ideals are generated by n elements
- Effects of Reconstitution of Membrane-Bound Lipoprotein Lipase and Dispersity of Substrate on Its Reaction Characteristics
- Spatial Properties of the Photoinduced Transition in Pr_Ca_MnO_3
- PJ-197 Left Ventricular Abnormal Relaxation is Induced by Reactive Oxygen Species in Dahl Salt Sensitive Rats(Heart failure, basic-6 (M) PJ33,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Calpain is Implicated in Rat Myocardial Injury after Ischemia or Reperfusion
- Emotional Stress Induces Transient Left Ventricular Hypocontraction in the Rat Via Activation of Cardiac Adrenoceptors : A Possible Animal Model of 'Tako-Tsubo' Cardiomyopathy
- IS063 MAP kinase activation and early immediate gene induction in the rat myocardium after psychological stress
- Distinct Gene Expression in the Stomach Following Stress and Alcohol Exposure
- PE-032 Up-regulation of Hemeoxygenase 1 in an Animal Model of Takotsubo Cardiomyopathy(Autonomic nervous system(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Author's Reply(Animal Model of 'Tako-Tsubo' Cardiomyopathy : Update)
- Correlation between Photoluminescence Intensity and Micro Structure in Amorphous Silicon Films Prepared by Reactive RF Sputtering
- PJ-148 Estrogen Replacement Suppresses Stress-induced Cardiovascular Responses in Ovariectomized Rats(Endothelium/NO 2 (H) : PJ25)(Poster Session (Japanese))
- A case of sudden death after intramuscular injection of butylscopolamine bromide
- Immobilization stress reduced the expression of neurotrophins and their receptors in the rat brain
- Intergral closedness in finitely generated ring-extensions of Noetherian domains
- Senescence marker protein-30 (SMP30) enhances the calcium efflux from renal tubular epithelial cells
- Phosphoinositide 3-Kinase Accelerates Calpain-Dependent Proteolysis of Fodrin during Hypoxic Cell Death
- Translocation of HSP27 and MKBP in Ischemic Heart
- Transient Up-regulation of Myotonic Dystrophy Protein Kinase-binding Protein, MKBP, and HSP27 in the Neonatal Myocardium
- Organization and Expression of the Bacillus subtilis sigY Operon
- Usefulness of blood vessels as a DNA source for PCR-based genotyping based on two cases of corpse dismemberment
- D-Pinitol and myo-Inositol Stimulate Translocation of Glucose Transporter 4 in Skeletal Muscle of C57BL/6 Mice
- Adrenomedullin and its Related Peptide
- Restraint Stress Induces Connexin-43 Translocation via α-Adrenoceptors in Rat Heart
- Identification of Two Major Ammonia-Releasing Reactions Involved in Secondary Natto Fermentation
- Differential Substrate Specificity of Two Inositol Transporters of Bacillus subtilis
- Altered structure and expression of Trk C gene on SHR and hypertensive substrains
- Expression Mechanism of Nerve Growth Factor Gene in Spontaneously Hypertensive Rats
- Over-expression of neurotrophins mRNA in the brain of stroke-prone spontaneously hypertensive rats
- 1 Preventive Effects of Estrogen and B Blocker on Stress-Induced Cardiac Dysfunction in a Experimental Rat Model(Is Stress a Risk of Cardiovascular Disease?,Symposium 22 (SY-22) (I),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Emotional stress of rats reduced the contraction of left ventricle-A possible animal model of "Takotsubo"-shaped cardiomyopathy
- P435 IMMOBILIZATION STRESS INDUCES EXPRESSION OF IMMEDIATE EARLY GENES IN THE HEART
- PJ-006 Brief Ischemia Enhances Gap Junction Intracellular Communication, Thereby Promoting Cell-to-Cell Spread of Cell Death in Rat Heart(Acute myocardial infarction, basic(02)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japane
- Stress-induced expression of immediate early genes in the brain and peripheral organs of the rat
- Determination of amobarbital and phenobarbital in serum by gas chromatography-mass spectrometry with addition of formic acid to the solvent
- Unexpected death of a patient with rheumatoid arthritis complicated by a cervical deformity
- 2 Experimental Findings of Takotsubo Disease(ESC-JCS Joint Symposium "Pathophysiology and Clinical Aspect of Takotsubo Disease", The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- 2Ap03 Bacillus subtilis cell factory for production of scyllo-inositol promising for Alzheimer's disease (Bio-Based Production)
- Production of Nerve Growth Factor in Cultured Vascular Smooth Muscle Cells from Genetically Hypertensive rats