Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene of Sinorhizobium fredii USDA191 Required for myo-Inositol Utilization
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概要
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Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently iolE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An iolE ortholog was cloned from USDA191. USDA191 iolE was expressed in Escherichia coli as a His6-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 iolE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis iolE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myo-inositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and iolE.
- 社団法人 日本農芸化学会の論文
- 2006-12-23
著者
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Mukai Rie
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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Yoshida Ken-ichi
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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ASHIDA Hitoshi
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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Ashida H
Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University
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Ashida Hitoshi
Research Center For Food Safety And Security Graduate School Of Agricultural Science Kobe University
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Ashida Hitoshi
Department Of Agricultural Chemistry Kobe University
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Ashida Hitoshi
Graduate School Of Science & Technology Division Of Life Science Kobe University
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Kinehara Masaki
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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KIM Won-Seok
Department of Agronomy, University of Missouri
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IKEDA Hideki
Department of Biotechnology, Fukuyama University
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FUJITA Yasutaro
Department of Biotechnology, Fukuyama University
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KRISHNAN Hari
Department of Agronomy, University of Missouri
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Ashida Hitoshi
Department Of Life Science Graduate School Of Science And Technology Kobe University
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Ashida Hitoshi
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University:research Center
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Ashida Hitoshi
Res. Center For Food Safety And Security Graduate School Of Agricultural Sci. Kobe Univ.
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Fujita Yasutaro
Department Of Biotechnology Faculty Of Engineering Fukuyama University
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Fujita Yasutaro
Department Of Biotechnology Fukuyama University
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Kim Won-seok
Department Of Agronomy University Of Missouri
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Krishnan Hari
Department Of Agronomy University Of Missouri
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Yoshida Ken-ichi
Dep. Of Agrobioscience Graduate School Of Agricultural Sci. Kobe Univ.
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Kinehara Masaki
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Yoshida Ken-ichi
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Mukai Rie
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Ikeda Hideki
Department Of Biotechnology Fukuyama University
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Mukai Rie
Dep. Of Food Sci. Graduate School Of Nutrition And Biosciences The Univ. Of Tokushima
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Yoshida Ken-ichi
Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University
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FUJITA Yasutaro
Department of Biochemistry, Hamamatsu University School of Medicine
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