Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts
スポンサーリンク
概要
- 論文の詳細を見る
We aimed to identify a novel flavonoid from the in-house natural products to suppress matrix metalloproteases (MMPs), which is responsible for degradation of collagen and other extracellular matrix proteins. Total eight natural products were screened for identification of a novel MMP-9 suppressor using MMP-9 reporter system, where the prompt initial screening with multiple samples is readily examined. Among the extracts used in the present study, one extract (Citrus unshiu) was found active in this assay system. Furthermore, three representative flavonoids in this active extract of Citrus unshiu peel were tested in MMP-9 reporter system. Nobiletin (NB) of the tested flavonoids suppressed MMP-9 expression without cytotoxicity, which was validated by both real-time polymerase chain reaction (PCR) and zymography analyses. Sustained p38 mitogen activated protein kinase (MAPK) activity, closely associated with induction of MMP-9 under stress condition, was markedly reduced by NB treatment, which implies that modulation of p38MAPK by nobiletin is responsible for reduction of MMP9 expression. Hence, nobiletin, identified from MMP-9 reporter system based screening, may be further applied for the purpose of delaying collagen degradation in skin fibroblasts.
著者
-
Chin Young-won
College Of Pharmacy And Research Institute Of Pharmaceutical Science Seoul National University
-
Kim Won-seok
Department Of Agronomy University Of Missouri
-
Chin Young-Won
College of Pharmacy, Dongguk University-Seoul
-
Cha Hyuk-Jin
Department of Life Science, College of Natural Science, Sogang University
-
Lee Ji-Seon
Department of Life Science, College of Natural Science, Sogang University
-
Kim Jin-Ju
Department of Life Science, College of Natural Science, Sogang University
-
Korm Sovannarith
Department of Life Science, College of Natural Science, Sogang University
-
Kim Ok-Seon
Department of Life Science, College of Natural Science, Sogang University
-
Min Hyung-Geun
Clean up Skin Clinic
-
Kim Won-Seok
Department of Dermatology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine
関連論文
- 熱帯地域のマレーシア住民が温帯地域の日本滞在による順化に伴なう発汗運動変異
- Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene of Sinorhizobium fredii USDA191 Required for myo-Inositol Utilization
- Two New Neolignans from the Aerial Parts of Rodgersia podophylla
- Two New Acylated Iridoid Glucosides from the Aerial Parts of Paederia scandens
- Three New Flavonol Glycosides from the Aerial Parts of Rodgersia podophylla
- P-569 BIOLOGICAL ACTIVITIES OF RODGERSINOL, A NEW INOS AND COX-2 INHIBITOR FROM RODGERSIA PODOPHYLLA
- Anti-inflammatory Diterpene from Thyrsanthera suborbicularis
- Cytotoxic Terpenes from the Stems of Dipterocarpus obtusifolius Collected in Cambodia
- Triterpenes with Cytotoxicity from the Leaves of Vernicia fordii
- Anti-allergic Flavones from Arthraxon hispidus
- Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts