Isolation of Novel Bacteria and Actinomycetes Using Soil-Extract Agar Medium(Environmental Biotechnology)

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概要

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• Novel bacteria were discovered using an isolation technique consisting of (i) selection of microorganisms that grew on soil-extract agar medium, but not on conventional media, and (ii) detection of small microbial colonies with a microscope. Three bacterial strains thus isolated were provisionally designated Shinshu-th1, -th2, -th3, and five actinomycete strains, Shinshu-MS-01, -02, -03, -04, -05, respectively. Sequence analysis of their 16S rDNA showed that th1 had 95-96% homology with three unculturable bacteria, and th2 had 96% similarity to Bradyrhizobium sp., one unculturable and one unidentified bacterial strain. A phylogenetic study indicated that both strains were α-Proteobacteria belonging to the order Rhizobiales and the family Bradyrhizobiaceae. Since they had low homology (96%) with their close relatives, it is possible that th1 and th2 belong to a new genus. The actinomycetes Shinshu-MS-02 and -03 had 95-96% homology with four strains of Actinomadura, -04 had 95-96% similarity to Streptosporangium and Microbispora, and -05 had 97-98% homology with three strains of Acrocarpospora, Herbidospora and Planotetraspora. According to the phylogenetic study, both 02 and 03 are possibly new species of Actinomadura, -04 of Streptosporangium, and -05 of Acrocarpospora. Shinshu-th3 and -MS-01 were identified as Mycobacterium cookii and Frankia sp., respectively, having 99% homology with these species.

• 社団法人日本生物工学会の論文
• 2005-05-25

著者

• KAJIURA Takayuki

  Pharmaceutical Research Laboratories, Ajinomoto Co., Inc.

• SUZUKI Motomasa

  Department of Pediatrics, Okazaki City Hospital

• Sen Kikuo

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

• Sen K

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University

• Sen Kikuo

  Department Of Bioscience And Biotechnology Shinshu University

• Fudou Ryosuke

  Microbiology Group Institute Of Life Sciences Ajinomoto Co. Inc.

• Tabuchi Akira

  Department Of Biology Pennsylvania State University

• Tabuchi Akira

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University

• FUDOU Ryosuke

  Institute of Life Science, Ajinomoto Co., Inc.

• JOJIMA Yasuko

  Institute of Life Science, Ajinomoto Co., Inc.

• SHIBAI Hiroshiro

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

• Jojima Yasuko

  Microbiology Group Institute Of Life Sciences Ajinomoto Co. Inc.

• HAMAKI TAKEFUMI

  Department of Bioscience and Biotechnology Faculty of Agriculture, Shinshu University

• Shibai Hiroshiro

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University

• Kajiura Takayuki

  Pharmaceutical Research Laboratories Pharmaceutical Company Ajinomoto Co. Inc.

• Kajiura Takayuki

  Pharmaceutical Research Laboratories Ajinomoto Co. Inc.

• Fudo Ryosuke

  Central Research Laboratories Ajinomoto Co. Inc.

• Suzuki Motomasa

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University

• Fudou R

  Institute Of Life Sciences Ajinomoto Co. Inc.

• Fudou Ryosuke

  Institute Of Life Science Ajinomoto Co. Inc.

• Hamaki Takefumi

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University

• Sen Kikuo

  Department of Bioscience and Biotechnology Faculty of Agriculture, Shinshu University

• Jojima Yasuko

  Microbiology Group, Institute of Life Sciences, Ajinomoto Co., Inc.

• TABUCHI Akira

  Department of Agricultural and Biological Chemistry, Shinshu University

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