Characterization of 3-Guanidinopropionate Amidinohydrolase from Pseudomonas aeruginosa and a Comparative Study with 4-Guanidinobutyrate Amidinohydrolase from another Pseudomonas
スポンサーリンク
概要
- 論文の詳細を見る
3-Guanidinopropionate amidinohydrolase, a new enzyme (EC class 3.5.3), was purified 220-fold from Pseudomonas aeruginosa PAO 1 grown on 3-guanidinopropionate. The enzyme was found to be essentially homogeneous on polyacrylamide gel electrophoresis. The molecular weight of the native enzyme was estimated to be 195, 000-215, 000. The subunit molecular weight was estimated to be 36, 000. The optimal pH was 9.0. The Km value for 3-guanidinopropionate was 45mM. Incubation of the enzyme with EDTA in potassium phosphate buffer, pH 7.0, at 40°C resulted in almost complete inactivation, and the inactive enzyme was specifically reactivated by Mn2+. Taurocyamine (11%) and 4-guanidinobutyrate (3%) were hydrolyzed as fast as 3-guanidinopropionate at the relative rates indicated. The enzyme was inactivated by p-chloromercuribenzoic acid and the inactive enzyme was reactivated by incubation with 2-mercaptoethanol. Coelectrophoresis of the enzyme with 4-guanidinobutyrate amidinohydrolase purified from Pseudomonas sp. ATCC 14676 in polyacrylamide gels in the presence and absence of sodium dodecyl sulfate demonstrated their close mobilities. 4-Aminobutyrate, propionate, and n-butyrate were common competitive inhibitors of these enzymes. The evolutionary relationship between the two enzymes was discussed.
- 社団法人 日本農芸化学会の論文
著者
-
Yorifuji Takamitsu
Department Of Agricultural And Biological Chemistry Shinshu University
-
Matsumoto Hideki
Department Applied Physics Seikei University:(present Address)institute Of Physics University Of Tsu
-
TABUCHI Akira
Department of Agricultural and Biological Chemistry, Shinshu University
-
SUGAI Ichiro
Department of Agricultural and Biological Chemistry, Shinshu University
-
YORIFUJI Takamitsu
Department of Agricaltural Chemistry, Faculty of Agriculture, Shinshu University
関連論文
- Thin Films of Ferromagnetic Superconductors
- Surface Magnetization in Ferromagnetic Superconductors
- Effective Exchange Interaction near the Surface of Ferromagnetic Superconductors
- Spin Wave Spectrum in the Spin-Spiral State of Ferromagnetic Superconductors
- Cell growth and morphology of Dictyostelium discoideum in space environment
- Cell growth and morphology of Dictyostelium discoideum in space environment
- Purification and Properties of Phenylethylamine Oxidase of Arthrobacter globiformis
- Radiation-Induced Adaptive Responses and Bystander Effects
- DNA Damage Formation and p53 Accumulation in Mammalian Cells Exposed to the Space Environment
- Primary central nervous system lymphoma secretes monocyte chemoattractant protein 1
- Taurocyamine-utilizing Mutant of Pseudomonas aeruginosa with Altered Induction of 3-Guanidinopropionate Amidinohydrolase(Microbiology & Fermentation Industry)
- THE VIABILITY OF LIVER GRAFT FROM FASTED DONOR IN RAT : FOCUSED ON THE RELATION WITH SINUSOIDAL ENDOTHELIAL CELL APOPTOSIS(THE SIXTH JAPAN-CHINA JOINT SEMINAR ON HISTOCHEMISTRY AND CYTOCHEMISTRY)
- 4-Aminobutyraldehyde and 4-Guanidinobutyraldehyde Dehydrogenases for Arginine Degradation in Pseudomonas putida(Biological Chemistry)
- NADP-Dependent Phenylacetaldehyde Dehydrogenase for Degradation of Phenylethyl-amine in Arthrobacter globiformis
- Phenylethylamine Oxidase, a Novel Enzyme of Arthrobacter globiformis, May Be a Quinoprotein(Biological Chemistry)
- Rare Bacterium of New Genus Isolated with Prolonged Enrichment Culture
- Isolation of Novel Bacteria and Actinomycetes Using Soil-Extract Agar Medium(Environmental Biotechnology)
- Nonequilibrium Formulation in Bose-Einstein Condensed States
- Mn^-Dependent Amidino-aspartase in Pseudomonas putida
- Effect on Liver and Serum Lipids in Rats of Dietary Additions of Fibers and Cholestyramine to a Cystine-excess Diet(Food & Nutrition)
- Biochemical Characterization of a Pseudomonas fluorescens Strain Isolated from a Benzalkonium Chloride Solution
- Histamine Oxidase, a Cu^-Quinoprotein Enzyme of Arthrobacter globiformis
- Plant Regeneration of Peppermint, Mentha piperita, from the Hairy Roots Generated from Microsegment Infected with Agrobacterium rhizogenes
- Alteration of Essential Oil Composition in Transgenic Peppermint (Mentha piperita) Carrying T-DNA from Agrobacterium rhizogenes
- Diguanidinobutane (Arcaine) Degradation in Rhodococcus sp. C-x
- Diaminopropane Aminotransferase, a Novel Enzyme of a Coryneform Bacterium(Biological Chemistry)
- Action of Polyamine Aminotransferase on Norspermidine
- Purification and Characterization of Polyamine Aminotransferase of Arthrobacter sp. TMP-1^1
- Xyloglucan Oligosaccharides Cause Cell Wall Loosening by Enhancing Xyloglucan Endotransglucosylase/Hydrolase Activity in Azuki Bean Epicotyls
- Action of Xyloglucan Hydrolase within the Native Cell Wall Architecture and Its Effect on Cell Wall Extensibility in Azuki Bean Epicotyls
- A New Type of Endo-Xyloglucan Transferase Devoted to Xyloglucan Hydrolysis in the Cell Wall of Azuki Bean Epicotyls
- Purification of Xyloglucan Hydrolase/Endotransferase from Cell Walls of Azuki Bean Epicotyls
- Guanidinobutyrase for L-Arginine Degradation in Brevibacterium helvolum
- Degradation of α, ω-Diguanidinoalkanes and a Novel Enzyme, Diguanidinobutane Amidinohydrolase in Pseudomonas putida(Microbiology & Fermentation Industry)
- Succinate-semialdehyde Dehydrogenase for L-Arginine and Putrescine Degradation in Brevibacterium helvolum(Microbiology & Fermentation Industry)
- Specific Measurement of Putrescine with Putrescine Oxidase and Aminobutyraldehyde Dehydrogenase(Biological Chemistry)
- Degradation of 2-Ketoarginine by Guanidinobutyrase in Arginine Aminotransferase Pathway of Brevibacterium helvolum
- Metabolism of L-Arginine, Agmatine, and Related Compounds in Nocardioides simplex
- Function of the Arginine Oxygenase Pathway in Utilization of L-Arginine-related Compounds in Arthrobacter globiformis and Brevibacterium helvolum
- Ultrastructure of Active Chromatin
- Differential Enzymatic Measurement of Agmatine, Putrescine, and Diguanidinobutane
- A Simple Approximation of Weight-based Protein Measurement with HPLC Apparatus(Biological Chemistry)
- Distribution of the Arginine Oxygenase Pathway among Coryneform Bacteria(Microbiology & Fermentation Industry)
- Construction of a Combined Notl/Smal Physical and Genetic Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238
- Magnetic Moments of Leptons and the Leptonic Boson
- Transition Temperature in One-Dimensional Bose-Einstein Condensation
- The Macroscopic Surface Phenomena and Topological Singularities in the Superconductivity
- Quantum Phase Coordinate as a Zero-Mode in Bose-Einstein Condensed States
- The Dynamical Rearrangement of Symmetry and the Group Contraction
- Phase Invariant Perturbation Scheme in Bose-Einstein Condensed States
- Ward-Takahashi Identities in Itinerant Electron Ferromagnetism.I : Zero Temperature Case
- Characterization of 3-Guanidinopropionate Amidinohydrolase from Pseudomonas aeruginosa and a Comparative Study with 4-Guanidinobutyrate Amidinohydrolase from another Pseudomonas
- Distribution of Amidinohydrolases among Pseudomonas and Comparative Studies of Some Purified Enzymes by One-dimensional Peptide Mapping
- Formation of 4-Hydroxy-3, 7-dimethyl-2, 6-octadienal (5-Hydroxycitral) from 3, 7-Dimethyl-2, 6-octadienal (Citral) and its Biological Activity against Sarcoma 180
- Gratuitous Induction of Two ω-Guanidino Acid Amidinohydrolases in Pseudomonas sp. ATCC 14676 by Taurocyamine and 3-Guanidinopropionate
- Action of Polyamine Aminotransferase on Norspermidine.
- Arginine Gene Cluster of Serratia marcescens
- A Generalized Transducing Phage of Serratia marcescens